2-(chloromethyl)-4-methylquinazoline

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-01-16 to 2007-03-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Version / remarks:

1996

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: samples from the test media of the test concentration and the control at the start and at the end of the test
- Sampling method: samples were taken from the approximate centre of the aquaria without mixing of the test media
- Sample storage conditions before analysis: All samples were analysed directly after sampling. The samples are kept stored in deep-freezer up to the date of the final report.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A stock solution with a concentration of 100 mg/L was prepared with test item and aquarium water. The test solutions were prepared by adding the appropriate volume of the stock solution to the appropriate amount of aquarium water so that the total volume of the test solutions was 5 L. The test media were freshly prepared just before the start of the test (= introduction of fishes).

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain: Brachydanio rerio
- Source: "Black Molly" Fish Farm, Veszprém, Hungary
- Age at study initiation (mean and range, SD): Juveniles
- Length at study initiation (length definition, mean, range and SD): 2.6 - 3.3 cm (on 10 animals measured)
- Weight at study initiation (mean and range, SD): 0.34 - 0.47 g
- Feeding during test: None

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: commercial fish diet for zebrafish
- Health during acclimation (any mortality observed): During the 12 days prior to the test no fish died in the test fish batch and all fish were healthy.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

167.4 mg CaCO3/L (determined before the start of the study)

Test temperature:

21.4 – 22.1 °C

pH:

8.34 - 8.49

Dissolved oxygen:

> = 95 %

Nominal and measured concentrations:

- Nominal: 0.125; 0.250; 0.500; 1.000; 2.000 and 4.000 mg test item/L
- Measured: 0.109; 0.231; 0.466; 1.032; 2.105 and 4.240 mg test item/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 8-litre glass aquaria
- Fill volume: 5 litre test medium
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, pH, Dissolved Oxygen and Water Temperature

TEST CONDITIONS:
- Observation time: 3, 6, 24, 48, 72 and 96 hours after start of the test

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

1.13 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 0.90 - 1.41

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.72 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 0.59 - 0.87

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

0.67 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 0.56 - 0.81

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.62 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL = 0.51 - 0.76

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1.032 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: nominal: 1.00

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

0.466 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: nominal: 0.50

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.231 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: nominal: 0.25

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.231 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: nominal: 0.25

Details on results:

- Behavioural abnormalities: Loss of equilibrium was observed in the test item treated groups of 0.466 (nominal 0.50); 1.032 (nominal 1.00) and 4.240 (nominal 4.00) mg/L. Spiral swimming was observed at the test item concentration of 0.466 (nominal 0.50) mg/L.

Sublethal observations / clinical signs:

Mortality:

Mortality occurred at the test item concentrations of 0.466 (nominal 0.50); 1.032 (nominal 1.00); 2.105 (nominal 2.00) and 4.240 (nominal 4.00) mg/L.

Analytical Results:

The analytically determined mean test item concentrations in the analysed test media varied in the range of 64 % to 110 % of the nominal value during the study. As the deviation of the measured concentrations from the nominal values was higher than 20 % in some cases, all reported biological results are related to the mean of the measured concentrations of the test item.

concentration [mg/L]				%-loss in the test item concentration over the test period
nominal	measured
	start	end	average
0.125	0.138	0.080	0.109	42.03
0.250	0.274	0.231	0.231	31.39
0.500	0.539	0.392	0.466	27.27
1.000	1.10	0.963	1.032	12.45
2.000	2.13	2.08	2.105	2.35
4.000	4.19	4.29	4.240	-2.39

Validity criteria fulfilled:

yes

Conclusions:

A 96-h LC50 value of 0.62 mg/L for Zebrafish was determined for the test item under the conditions of the test.

Description of key information

A 96 -h LC50 value of 0.62 mg/L to zebrafish was determined for the substance under the conditions of the test. It was determined in a reliable study conducted according to an appropriate standard tes method (OECD 203).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.62 mg/L