α,α,α,2-tetrachlorotoluene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

α,α,α,2-tetrachlorotoluene

EC Number:

218-377-7

EC Name:

α,α,α,2-tetrachlorotoluene

Cas Number:

2136-89-2

Molecular formula:

C7H4Cl4

IUPAC Name:

1-chloro-2-(trichloromethyl)benzene

Details on test material:

Purity: 97.0%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG, Kastengrund, SPF-Zucht
- Age at study initiation: no data
- Weight at study initiation: males: 179 g - 202 g (average 191 g); females: 173 g - 204 g (average 188 g)
- Fasting period before study: about 16 hours
- Housing: 5 animals per makrolon cage
- Diet: Altromin 1324 rat diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 50 +/- 20%
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Sesame oil

Details on oral exposure:

- Concentration in vehicle: 10,0, 12.5, 14.0, 16.0, 20.0%
- Dose volume applied: 10.0 ml/kg bw

Doses:

1000, 1250, 1400, 1600, 2000 mg/kg bw

No. of animals per sex per dose:

5

Details on study design:

- Duration of observation period following administration: 14 days and 21 days for the group of female rats exposed to 1600 mg/kg bw.
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology, histopathology

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

1 270 mg/kg bw

Based on:

test mat.

95% CL:

ca. 1 050 - <= 1 560

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

1 490 mg/kg bw

Based on:

test mat.

95% CL:

>= 1 210 - <= 1 750

Effect levels 3

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 380 mg/kg bw

Based on:

test mat.

95% CL:

>= 1 220 - <= 1 560

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The LD50 value for acute oral toxicity is 1270 mg/kg bw in male Wistar rats and 1490 mg/kg bw in female Wistar rats.