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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
not biodegradable
Type of water:
freshwater

Additional information

The Ready Biodegradability of the test item was determined in a closed bottle test in accordance with OECD 301D at a concentration of 2 mg/L in mineral medium inoculated with secondary effluent from a treatment plant (1 mL/L). Sodium benzoate was used as a reference substance to check the validity of the method. Mineral medium with inoculum (1 mL/L) without test item and reference substance served as an inoculum blank. Separate test solutions were maintained in parallel (in duplicate) for test, reference, and control samples for observation on day 0, 7, 14, 21, 28 and 35.The ThOD value for the test and reference item was 2.094 mg O2/mg and 1.666 mg O2/mg, respectively.


The percentage biodegradation of the test item in test suspension and toxicity control, revealed that the test item was not readily biodegradable and not inhibitory to microbial activity in the inoculum. The maximum degradation of the test item was 14.23% on day 35. The percent degradation in the toxicity control was greater than 25% after 14 days.