Hexane-1,3,6-tricarbonitrile

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

effects on growth of green algae

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2022.08.23-2022.10.13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Green alga
- Strain: SAG 61.81

ACCLIMATION
The algal Suspension in breeding in Eurofins Biolab S.r.l has been kept in culture medium in sterile 250
mL Erlenmeyer flasks in the following conditions until the beginning of the test:
Temperature: 23 ± 2℃
Lighting: >4300 lux
Stirring: steadu with ES.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

pH:

8.15

Dissolved oxygen:

23℃

Nominal and measured concentrations:

100 mg/L

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Validity criteria fulfilled:

yes

Conclusions:

The obtained results, in compliance with assay validity criteria, showed that Algae ECso of the concentration of the test item ‘HEXANE-1,3,6-TRICARBONITRILE” after 72 hours is > 100 mg/L.

Executive summary:

During the range finding test the algae have been exposed to 5 different dilutions of the test item for 72
hours. After the results of the range finding test a limit test has been performed. The algae have been
exposed to 100 mg/L of the test item for 72 hours. Algae have been measured using a Burker chamber.
At the end of the test, microscopic observation was performed to verify a normal and healthy
appearance of the inoculum Culture and to observe any abnormal appearance of the algae.
At the beginning and at the end of the test (72 hours) pH has been measured in all the vessels.
Temperature of the ambient has been recorded every day.

The obtained results, in compliance with test validity criteria, can be Summarised as follows:
EC50 (72 h) of the test item ‘HEXANE-1,3,6-TRICARBONITRILE”. > 100 mg/L
% Ir (inhibition in average specific growth rate) = 1.96%

Reference 2

Endpoint:

effects on growth of green algae

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Guideline:

other: REACH guidance on QSARs R.6

Principles of method if other than guideline:

General model

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

5.28 mg/L

Conclusions:

72h EC50=5.28 mg/L was predcited.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

EC10 or NOEC for freshwater algae:

100 mg/L