N,N,N-tri-C8-C10-alkyl-N-methyl-1-aminium sulfate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-12-10 to 2019-12-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

2008

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

A stock solution (100 mg/L of the test item were weighed out) was freshly prepared with natural river water (Table 3, diluted by factor 2 with demineralized water) prior to the start of the exposure (at 0 hours) and prior to the renewal of the test solutions (at 24 hours). The stock solution was treated with ultrasound for 5 minutes at room temperature and mixed thoroughly by manual agitation.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna STRAUS
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age at study initiation (mean and range, SD): 2 to 24 hours old daphnids
- Method of breeding: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2°C, in an incubator, 16 h illumination, light intensity of max. 1500 lx
- Feeding during test: no

ACCLIMATIZATION
- Acclimatization period: At least 2 hours in river water
- Acclimatization conditions (same as test or not): same
- Type and amount of food: culture daphnids are fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus. The algae are cultured at the test facility.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

river water: Total hardness [mg CO3/L]: 347
Dilution Water: Time 0 h: Total hardness [mg CO3/L]: 158
Dilution Water: Time 24 h: Total hardness [mg CO3/L]: 156

Test temperature:

18 - 22 °C, constant within ± 1 °C

pH:

start: 7.62 - 7.82 (test concentrations: 1.41 - 32.3 mg/l), 7.78 (control); end: 7.03 - 7.89 (test concentrations: 1.41 - 32.3 mg/l) 7.36 (control)

Dissolved oxygen:

start: 8.64 - 8.77 mg/l (test concentrations: 1.41 - 32.3 mg/l), 8.77 mg/l (control); end: 8.05 - 8.35 mg/l (test concentrations: 1.41 - 32.3 mg/l) 8.31 mg/l (control)

Conductivity:

502 µS/cm (0 h), 481 µS/cm (24 h)

Nominal and measured concentrations:

1.41, 2.91, 5.21, 13.4, 32.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20  2 °C, 16 hours illumination, light intensity of max. 1500 lx
- Renewal rate of test solution (frequency/flow rate): renewal of the test solutions at 24 hours
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
river water as specified in section "any other information on materials and methods"

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light; light intensity of max. 1500 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Immobilisation was determined in all groups after 24 and 48 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Range finding study
- Test concentrations: 0.01, 0.1, 1 and 10 mg/L, prepared in natural river water
2 replicates with 5 daphnids each
- Results used to determine the conditions for the definitive study: immobilisation at 0.01 and 0.1 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

28.1 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- immobilisation of test item: 65 % (mean value at 48 h and highest conc. 32.3 µg/L)
- immobilisation of control: 0 %

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 2.03 mg/L (CI 1.00 - 4.00 mg/L)

Reported statistics and error estimates:

The respective confidence limits were empirically derived from the observation data as follows: The highest concentration level without any effect (EC0) and the lowest concentration level causing 100% immobilization (EC100) were used as confidence limits.

The percentage of immobility, determined in concentration levels of the test item and in the control after 24 and 48 hours is given inTable 4. The absolute numbers of immobile daphnids are presented in Table 5.

 Table 4: Immobilization Ratesafter 24 and 48 hours of Exposure in the Definitive Test

           (n = 20, divided into 4 replicates with 5 daphnids each)

Geometric mean measured concentration of the test item [µg/L]	IMMOBILIZATION [%]
	24 hours					48 hours
	Replicates					Replicates
	1	2	3	4	MV	1	2	3	4	MV
32.3	0	0	60	20	20	60	20	100	80	65
13.4	0	0	0	0	0	0	0	0	0	0
5.21	0	0	0	0	0	0	0	0	0	0
2.91	0	0	0	0	0	0	0	0	0	0
1.41	0	0	0	0	0	0	0	0	0	0
Control	0	0	0	0	0	0	0	0	0	0

Table 5: Absolute Numbers of immobileDaphnids after 24 and 48 hours ofExposure in the Definitive Test

           (n = 20, divided into 4 replicates with 5 daphnids each)

Geometric mean measured concentration of the test item [µg/L]	Number of immobile Daphnids / Total number of Daphnids
	24 hours					48 hours
	Replicates					Replicates
	1	2	3	4	Sum	1	2	3	4	Sum
32.3	0 / 5	0 / 5	3 / 5	1 / 5	4 / 20	3 / 5	1 / 5	5 / 5	4 / 5	13 / 20
13.4	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20
5.21	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20
2.91	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20
1.41	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20
Control	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20	0 / 5	0 / 5	0 / 5	0 / 5	0 / 20

 

The effect levels (EC_{10 / 50 / 100}), based on the geometric mean measured concentrationsofthe test item are presented in Table 1.

Table 1:    EC₁₀-, EC₅₀- (with Confidence Limits) and EC₁₀₀-Values

                (based on the geometric mean measured concentrations of the test item)

Effect values	Test duration [hours]	Test item
		Geometric mean measured concentrations of the test item [µg/L]
EC10 (with confidence limits)	24	30.3 (Cl: 6.76─> 32.3)
	48	28.1 (Cl: 6.01─> 32.3)
EC50 (with confidence limits)	24	> 32.3 (Cl: Not applicable)
	48	31.3 (Cl: 6.85─> 32.3)
EC100	24	> 32.3
	48	> 32.3

 

Validity criteria fulfilled:

yes

Conclusions:

Based on the geometric mean measured concentrations, the 48 hours-EC50 for Daphnia magna was 31.3 µg/L (95% confidence limits: 6.85 – > 32.3 µg/L) for the test item.

Executive summary:

The 48-h-acute toxicity of Quarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfates to Daphnia magna was studied under static conditions according to OECD Guideline 202 (2004).  Daphnids were exposed to control and test chemical at 5 concentrations (1.41, 2.91, 5.21, 13.4, 32.3 mg/L) in natural river water for 48 h.

Mortality/immobilization was observed daily. 

The 48 hours-EC10 for Daphnia magna was 28.1 µg/L (95% confidence limits: 6.01 – > 32.3 µg/L) for the test item.

The 48 hours-EC50 for Daphnia magna was 31.3 µg/L (95% confidence limits: 6.85 – > 32.3 µg/L) for the test item.

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.

 

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

31.3 µg/L

Additional information

The 48-h-acute toxicity ofQuarternary ammonium compounds, tri-C8-C10-alkylmethyl, Me sulfatesto Daphnia magna was studied under static conditions according to OECD Guideline 202 (2004).  Daphnids were exposed to control and test chemical at 5 concentrations (1.41, 2.91, 5.21, 13.4, 32.3mg/L) in natural river water for 48 h.

Mortality/immobilization was observed daily. 

The 48 hours-EC10 for Daphnia magna was 28.1 µg/L (95% confidence limits: 6.01 – > 32.3 µg/L) for the test item.

The 48 hours-EC50 for Daphnia magna was 31.3 µg/L (95% confidence limits: 6.85 – > 32.3 µg/L) for the test item.

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.