Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 807-577-5 | CAS number: 1182844-21-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November 8, 2012 - February 19, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- adopted March 23, 2006
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Council Regulation (EC) No. 761/2009 laying down test methods pursuant to Regulation (EC) No. 1907/2006 of the European Parliament and the council on the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH).
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- The test material concentration in the reconstituted water was not quantified at the start and the end of this study due to the low water solubility (< 0.051 mg/L). Because of the low water solubility, the compound cannot be detected with standard analytical methods. The development of an analytical method with a sufficiently low detection and quantification limit is complex. Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
- Vehicle:
- yes
- Details on test solutions:
- The test medium (reconstituted water and test item) was prepared freshly. Therefore, the calibrated flask with test medium was treated in an ultrasonic device for 1 hour. Subsequently, the preparation was stirred with a magnetic stirrer for further 23 hours. Afterwards, the formulation was passed through a nutsch filter with a pore size of 10-20 µm. The filtrate was used for the study.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Desmodesmus subspicatus
- Source (laboratory, culture collection): laboratory
- Age of inoculum (at test initiation): freshly prepared
- Method of cultivation: standard
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same
- Any deformed or abnormal cells observed: none - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- -
- Hardness:
- 250 mg/L
- Test temperature:
- 23 °C
- pH:
- 7.95 - 8.91
- Nominal and measured concentrations:
- nominal: 100.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 6 replicates
- Type (delete if not applicable): open
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
mg/L
NH4Cl 15.00000
MgCl2 * 6 H2O 12.00000
CaCl2 * 2 H2O 18.00000
MgSO4 * 7 H2O 15.00000
KH2PO4 1.60000
FeCl3 * 6 H2O 0.06400
Na2EDTA * 2 H2O 0.10000
H3BO3 0.18500
MnCl2 * 4 H2O 0.41500
ZnCl2 0.00300
CoCl2 * 6 H2O 0.00150
CuCl2 * 2 H2O 0.00001
Na2MoO4 * 2 H2O 0.00700
NaHCO3 50.00000
The pH of the reconstituted water after aeration is approximately 8. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: > 0.051 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: > 0.051 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: > 0.051 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: > 0.051 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: > 0.051 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: > 0.051 mg/L
- Results with reference substance (positive control):
- The sensitivity of the test system has to be demonstrated periodically using potassium dichromate.
Under the given experimental conditions, the test material Art. 104864 (potassium dichromate) showed 72 h EC values which are within the recommended range (Yield EC50 0.20 – 0.75 mg/L and Growth rate EC50 of 0.60 –1.03 mg/L). - Validity criteria fulfilled:
- yes
- Conclusions:
- An aqueous preparation of 100 mg/L (nominal) of the test item revealed no toxic effect in the test system. The 72-hours EC50 and EC10 could not be determined, because it exceeded the maximum solubility of the test material in reconstituted water (EC50 > 0.051 mg/L).
- Executive summary:
The objective of this study was to determine the influence of the test item on the growth and growth rate of the unicellular green algal species Desmodesmus subspicatus. The study design included one control group and one test material group with six replicates, each containing 100 mL reconstituted water or test medium and about 10000 cells/mL at the start of the experimental phase.
The study was performed as a limit test in an open static test system. The algae were exposed to s filtrate of nominal 100 mg test material per L. The growth of the algae was calculated after 24, 48, and 72 hours exposure in the test item and control groups.
This study was performed according to GLP and the methods applied are fully compliant with OECD TG 201.
The test material concentration in the reconstituted water was not quantified at the start and the end of this study due to the low water solubility (< 0.051 mg/L). Because of the low water solubility, the compound cannot be detected with standard analytical methods. The development of an analytical method with a sufficiently low detection and quantification limit is complex. Due to the absence of any adverse effects at the saturation concentration, the study was performed without analytical concentration verification.
EC values:
For the test item, the following values for Desmodesmus subspicatus were determined:
Parameter (0-72 h) Growth rate Yield EC10 > 100 mg/L (nominal) > 100 mg/L (nominal) EC20 > 100 mg/L (nominal) > 100 mg/L (nominal) EC50 > 100 mg/L (nominal) > 100 mg/L (nominal) NOEC > 100 mg/L (nominal) > 100 mg/L (nominal) LOEC > 100 mg/L (nominal) > 100 mg/L (nominal) An aqueous preparation of 100 mg/L (nominal) of the test item revealed no toxic effect in the test system. The 72 hours EC50 and EC10 could not be determined, because it exceeded the maximum solubility of the test material in reconstituted water (EC50 > 0.051 mg/L).
Reference
Description of key information
No adverse effects on algae were observed up to the limit of water solubility. Thus, the 72 -h EC50 value and 72 -h EC10 value are greater than 0.051 mg/L which corresponds to a nominal concentration of 100 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.