Alkenes, C6-11 (branched), hydroformylation products, distn. residues, heavy cracked fraction

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The reliability rating is a 2 because although the study did not follow a standard guideline (which did not exist for this type of study), it did follow valid scientific principles appropriate for this type of study, and the results were reviewed for reliability and assessed as valid. This study follows a standard protocol that was later agreed to by EU regulators in 2004 and is currently in draft form as part of an update to OECD TG 305.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2008

Reference 2

Reference Type:

grey literature

Title:

Fish dietary bioaccumulation study, basic protocol

Author:

Anonymous

Year:

2004

Bibliographic source:

Document prepared for the TC NES Subgroup on identification of PBT and vPvB substances

Reference 3

Reference Type:

publication

Title:

Guidance for Evaluating In Vivo Fish Bioaccumulation Data

Author:

Thomas F Parkerton, Jon A Arnot, Anne V Weisbrod, Christine Russom, Robert A Hoke, Kent Woodburn, Theo Traas, Mark Bonnell, Lawrence P Burkhard, and Mark A Lampi

Year:

2008

Bibliographic source:

Integrated Environmental Assessment and Management. 4:139-155

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

This study was performed to determine the half-life and elimination rate constant for the test substance from rainbow trout (Oncorhynchus mykiss) tissue. The exposure to test substance was through the diet, which included a 14-day uptake phase that was followed by a 21-day depuration phase. Results are based on analyses of fish tissue on day 14 of the uptake phase, followed by days 0, 1, 3, 7, 14 and 21 of the depuration phase.

Water quality results for some of the water batches had one pH value and several hardness values outside of the recommended ranges. Because there was no mortality in the study, those waters are not believed to have adversely affected the study.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Alkenes, C6-11 (branched), hydroformylation products, distn. residues, heavy cracked fraction

EC Number:

701-314-7

Molecular formula:

CnH2n+2O2. n=24-33

IUPAC Name:

Alkenes, C6-11 (branched), hydroformylation products, distn. residues, heavy cracked fraction

Details on test material:

Commercial substance

Radiolabelling:

no

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Organisms were randomly selected and sequentially placed directly to the test chambers until each of 2 chambers (control and exposure diet) contained 45 organisms. To ensure that quality organisms were used for the study, fish were selected from a pool of organisms larger than that needed for the study. The study director or his designee determined organism suitability.

The fish used in this study were fed at a level of approximately 3% of their wet body weight per day. The amount of feed was adjusted at each fish-sampling period to account for the growth of the fish during the experiment and the reduced number of fish in the test chambers.

The test organisms were quarantined and observed for parasites and disease for at least 7 days prior to use in the test, and were held in dilution water at approximately 15 degrees C, that was continuously aerated to provide a dissolved oxygen concentration of at least 80% of the air saturation value.
Fish were not treated for disease or parasites before use in this study. Fish were held under static conditions using biological and mechanical filtration, and were fed finfish starter (Zeigler Bros, Inc., Gardners, PA).

Study design

Route of exposure:

feed

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

14 d

Total depuration duration:

21 d

Test conditions

Details on test conditions:

A flow through system was used to provide a sufficient volume of dilution water to the test tanks. The flow rate was between 160 - 200 mL/minute.

The appropriate amounts of the test substance was added to the test feed to achieve nominal concentrations . A single diet was prepared containing the test substance. Untreated food was fed to the control fish. The treated and untreated diets were measured daily and fed to the fish as either one or two feedings.

Nominal and measured concentrations:

Nominal feed concentration: 502 ug/g feed. Mean measured concentration: 475 ug/g.

Reference substance (positive control):

no

Results and discussion

Lipid content

Lipid content:

5 %

Time point:

other: average of Day 0 depuration and Day 21 depuration.

Remarks on result:

other: % lipid not significantly different depuration 0d & 21d

Bioaccumulation factor

Conc. / dose:

475 µg/g food

Type:

BMF

Value:

1.43 dimensionless

Basis:

normalised lipid fraction

Calculation basis:

kinetic, corrected for growth

Rate constants

Rate constants 1

Rate constant:

growth-corrected half-life (d)

Value:

84.4

Rate constants 2

Rate constant:

growth-corrected depuration rate constant (d-1)

Value:

0.008

Any other information on results incl. tables

Substance is complex mixture. GC analysis of fish tissue allowed for elucidation of a peak of the substance. The BMF of the peak was 1.43.

Applicant's summary and conclusion

Conclusions:

The lipid corrected BMF(lipid) of the test substance was 1.43, with a half-life of 84.4 d.

Executive summary:

The lipid corrected BMF(lipid) of the test substance was 1.43, with a half-life of 84.4 d.