Dibenzoyl-L-tartaric acid monohydrate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05/09/2018 - 17/09/2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Key result

Water solubility:

3.2 g/L

Conc. based on:

test mat.

Incubation duration:

72 h

Temp.:

20 °C

pH:

>= 2.14 - <= 2.15

Table 1. Results of test item solubility in water

Mixing time at 30 ºC [days, flasks]	1		2		3
Samples	1	2	3	4	5	6
Temperature, ºC	19.8		19.9		19.7
pH	2.14		2.15		2.15
Concentration of test item, g/kg	3.2220	3.1934	3.2392	3.2371	3.1585	3.1501
Average concentration of test item, g/kg	3.2077		3.2382		3.1543
Average concentration test item g/kg	3.2001

 

Table 2. Results of the preliminary test

0.1g in x mL of solution	0.1	0.5	1	2	10	100	> 100
Soluble (+/-)	-	-	-	-	-	+
Approximate solubility, g/L	> 1000	1000 to 200	200 to 100	100 to 50	50 to 10	10 to 1	< 1

Table 3.Calibration solution

Day	Weight [mg]	Dilution [mL]	Cw [mg/mL]	Peak area		Average peak area Aw	f	Average f
1	10.58	25-0.5-10-1-10	0.00211	156823	157652	157237.5	0.00001339	0.00001292
		25-0.8-10-1-10	0.00337	265192	276252	270722	0.00001245
2	10.58	25-0.5-10-1-10	0.00211	230154	228058	229106	0.00000947	0.00000913
		25-0.8-10-1-10	0.00337	392228	396971	394599.5	0.00000879
3	10.58	25-0.5-10-1-10	0.00211	170196	170396	170296	0.00001237	0.00001210
		25-0.8-10-1-10	0.00337	284291	285084	284687.5	0.00001184

Table 4. Study solution in water

Sample day/ flask		Weight of the sample [mg]	Dilution [mL]	Cp [mg/mL]	Peak area		Average peak area Ap	X [g/kg]
1	1	103.31	10-1-10	1.0331	255402	259898	257650	3.2220
	2	102.68		1.0268	253473	254136	253804.5	3.1934
2	3	102.07	10-1-10	1.0207	362322	361844	362083	3.2392
	4	102.36		1.0236	363037	362730	362883.5	3.2371
3	5	102.45	10-1-10	1.0245	268101	266735	267418	3.1585
	6	103.14		1.0314	264918	272082	268500	3.1501

 

Conclusions:

The water solubility of the test item at 20ºC is 3.20 at 20ºC  and a pH range of 2.14 to 2.15.

Executive summary:

The water solubility study of the test item using the flask method was performed in accordance with the OECD 105 guideline / Method EU A.6, under GLP conditions. After a preliminary test to determine the necessary concentration to saturate 70 mL of water, three 100 ml beakers were filled with 5 times this concentration. The three flasks were centrifuged for one day at 20ºC, and two and three days at 30ºC respectively. The concentration of the test item in each solution was determined by a validated HPLC method. Two replicates were performed; since results of the three vessels did not differ by more than 15% the test was considered satisfactory. The water solubility of the test item at 20ºC was found to be 3.20 g/L (pH 2.14 - 2.15).

Description of key information

Key study. Method according to OECD 105/ EU A.6, GLP study. The water solubility of the test item at 20ºC is 3.20 g/L.

Key value for chemical safety assessment

Water solubility:

3.2 g/L

at the temperature of:

20 °C