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EC number: 947-697-6 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From 2010-04-07 to 2010-06-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- Deviations:
- no
- Principles of method if other than guideline:
- Not relevant.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed on June 23, 2010
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in th emineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day. - Duration of test (contact time):
- 42 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- the substance samples (10.0 mg; corresponding to 100 mg/l in 100 ml of test medium) are weighed in small aluminium boats and added directly to the test flasks of the Oxitop, whereas the substance reference (sodium benzoate) is added as 1.00 ml of a 10.0 g/L solution in mineral medium, to give a total volume of 101 ml.
Flasks are filled with 97 ml of mineral medium (flasks containing sludge only; 194 ml). Samples of the test reference substance are added.. then suspended sludge diluted to a concentration of 1.0 g/l dry matter is added. Except when the test substance has an acid or alkaline character, the pH of each flask is not measured but assumed to be the same as the mineral medium, in order to not remove any floating undissolved test substance from the test medium by dipping a glass electrode in it. Neutral test substances, even sodium benzoate, were shouwn not to affect pH of the medium by more than 0.1 pH unit. Two sodium hydroxide pellets are placed in the quivers on top of the bottle, and the flasks are closed tightly with the measured heads. The flasks are cooled to about 18 - 20 °C. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L
- Preliminary study:
- None.
- Test performance:
- No data.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 87
- Sampling time:
- 28 d
- Details on results:
- Day 3: 23% degradation
Day 13: 77% degradation
Day 42: 91% degracation - Results with reference substance:
- Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Carvacrol should be regarded as readily biodegradable according to this test.
- Executive summary:
The ready biodegradability of Carvacrol was determined by the Manometric Respirometry test.
Carvacrol undergoes 87% biodegradation after 28 days (91% after 42 days) in the test conditions. The 10 -days window criterion is also fulfilled ( 23% biodegrdation on day 3 and 77% on day 13).
Thus Carvacrol should be regarded as readily biodegradable according to this test.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1996
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to OECD Guideline 301 C but with deviations: no detail on material and methods
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- yes
- Remarks:
- no detail on material and methods
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- No data
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Concentration of sludge: 30 mg/L
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Details on study design:
- No data
- Preliminary study:
- No data
- Test performance:
- No data
- Parameter:
- % degradation (O2 consumption)
- Value:
- >= 90 - <= 99
- Sampling time:
- 28 d
- Details on results:
- - Indirect analysis (BOD): 90%
- Direct analysis (TOC) & (GC): 99% and 100% respectively. - Results with reference substance:
- No data
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the test conditions, linalool was readily biodegradable.
- Executive summary:
In a ready biodegradation study performed according to OECD Guideline 301 C, linalool was tested at concentrations of 100 mg/L and the inoculum was activated sludge (30 mg/L). The degradation of the test material was assessed by the determination of the oxygen consumption.
At 100 mg/L test concentration, 90 -99% degradation (biochemical oxygen demand) was reached in 28 days.
Under the test conditions, linalool was readily biodegradable.
Referenceopen allclose all
The 10-day window criterion is fulfilled.
None
Description of key information
Based on a weight of evidence approach with data on constituents, the registered substance can be considered as readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
According to the SIP of the substance, constituents carvacrol and linalool represents 73.7% of the substance composition. Both constituents are considered as readily biodegradable.
During tests, carvacrol and linalool were found to be degraded after 28 days at 87% and 90% under the 10 days window, respectively.
Based on these elements, the registered substance Oregano Essential Oil is expected to be degraded at more than 60% after 28 days during a screening test.
Thus, the Oregano essential Oil is considered as readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
