N,N,N',N'-tetramethylethylenediamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1 - 5 February 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

No chemical analysis was performed.

Qualifier:

according to guideline

Guideline:

other: USEPA TSCA 40 CFR Part 797.1400, 9/27/85, 5/20/87

Deviations:

not specified

GLP compliance:

yes

Specific details on test material used for the study:

TMEDA Lot number #7-0906
Clear, pale-yellow, water miscible liquid with strong ammonia odour

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

not specified

Remarks:

Material described as water miscible so vehicle not likely to have been used.

Details on test solutions:

Not details reported

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Obtained from Musky Trout hatchery, Asbury, NJ.
Fish hatched 10 March 1987 (approx)
Tested age 121 days, less than 5% mortality during acclimation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

Not applicable

Post exposure observation period:

Not applicable

Hardness:

Ranged between 90 and 100 mg/L as CaC03 over the duration of the study

Test temperature:

12°C

pH:

Measured pH was concentration dependent: after 24 hours pH was 7.4, 7.8, 8.0, 8.5, 9.2 and 9.5 in the 0, 100, 180, 320, 560 and 1000 mg/L treatments respectively. From 48 hours until the end of the study pH ranged from 7.7 to 8.4 in the 0, 100, 180 and 320 mg/L treatments.

Dissolved oxygen:

Ranged between 10.4 and 11.0 mg/L over the duration of the study

Salinity:

Not applicable

Conductivity:

Ranged between 200 and 300 (no units provided) over the duration of the study

Nominal and measured concentrations:

Nominal exposure concentrations - 0 (control), 100, 180, 320, 560 and 1000 mg/L

Details on test conditions:

Test vessels were 4000 mL non-plasticised polypropylene containers, each containing 3000 mL of test media.
Three replicate test vessels were used test treatment, each containing 7 fish.
Aeration was supplied to each test vessel using an oil-free supply at a rate of 100 +/- 10 bubbles per minute through a pipette
Temperature, dissolved oxygen, pH and conductivity were measured daily; hardness and alkalinity were measured at the start of the study.
The study was performed with a 16 hour light and 8 hour dark cycle using fluorescent lights between 100 and 200 ft-candles.
Fish were observed for mortality and various non-lethal effects.
A sample of 25 fish had a mean weight of 528 mg, a mean length of 38.7 mm ranging from 31 – 45 mm) and a loading factor of 1.2 g of fish/L.

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

240 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality after 96 hours was 0, 0, 0, 100, 100 and 100% in the control, 100, 180, 320, 560 and 1000 mg/L treatments.
There was a consistent trend of increasing mortality with time evident in the 320 mg/L treatment. Remaining concentrations had an "all or nothing" response.
No sub-lethal effects were observed in the control, 100 or 180 mg/L treatments during the study.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

The LC50 was calculated using the modified Litchfield-Wilcoxon method.

Sublethal observations / clinical signs:

Cumulative mortality

Nominal concentration (mg/L)	Number of fish*	Cumulative mortality (%)
		24 hours	48 hours	72 hours	96 hours
Control	21	0	0	0	0
100	21	0	0	0	0
180	21	0	0	0	0
320	21	86	95	100	100
560	21	100	100	100	100
1000	21	100	100	100	100

*Total of three replicates

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour LC50 for rainbow trout exposed to TMEDA was 240 mg/L, the lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.

Executive summary:

Rainbow trout (Salmo gairdneri) were exposed to TMEDA at nominal test concentrations of: 0, 100, 180, 320, 560 and 1000 mg/L. This study was carried out to USEPA TSCA 40 CFR Part 797.1400, 9/27/85, 5/20/87.  The study was performed as a static study with three replicates each containing seven fish, without analytical verification. 

After 96 hours mortality was 0, 0, 0, 100, 100 and 100% in the nominal 0 (control), 100, 180, 320, 560 and 1000 mg/L. The 96 hour LC50 for rainbow trout exposed to TMEDA was 240 mg/L, the lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.

Description of key information

The short-term toxicity of TMEDA to fish was determined in a 96-hour static test performed according to USEPA TSCA 40 CFR Part 797.1400 Guideline (2011) and in compliance with GLP.  The 96-h LC50 was 240 mg TMEDA/L.  The lowest concentration causing 100% mortality was 320 mg/L and the highest concentration causing no mortality was 180 mg/L. All concentrations are reported as nominal concentrations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

240 mg/L