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Diss Factsheets
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EC number: 701-266-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20-03-1990 to 25-03-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Study is complete, performed under GLP and valid. however no analysis was performed and thus the usability for riskassessment purposes is limited when assessing the bioavailable fraction.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out with the unicellular green algae; Selenastrum capricornutum ATCC 22662. This strain was maintained on mineral salts agar slants in the light at room temperature.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- between 22 and 24 °C
- pH:
- 7.1-8.8
- Nominal and measured concentrations:
- nominal: 0, 0.125, 0.250, 0.500, 1.000, 2.000 mg/L
- Details on test conditions:
- The culturing apparatus is a cabinet in which the temperature is maintained between 22° and 24°C, and continuous uniform illumination is provided in the spectral range of 400 to 700 nm. The light intensity is in the range of 6000 to 10000 lux which is obtained by using seven 30 W fluorescent lamps (colour temperature of approximately 4300 K), at a distance of 0.35 m from the algal culture. The culture flasks were rotated continuously at 100 rpm to prevent sedimentation of the algae.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: both growth rate and biomass
- Details on results:
- The ErC50 and EbC50 (0-72 h) of pentaethylenehexamine are 1. 7 and 0.7 mg/litre respectively. The NOEC determined of pentaethylenehexamime is 0.250 mg/litre.
- Results with reference substance (positive control):
- in line with guideline
- Reported statistics and error estimates:
- The EC50 values were computed from the best fitted line (least square method) through the points given by the probit of the percentage inhibition and the logarithm of the concentration of the test substance. The EC50 value calculated with the area under the growth curve is termed EbC50 (0-72 h) whereas the EC value calculated with the specific growth rate is termed ErC50 Confidence limits were computed based on Fieller's theorem. All computations were performed with the software package SAS.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 and EbC50 (0-72 h) of PEHA are 1.7 and 0.7 mg/litre respectively. The NOEC determined of PEHA is 0.25 mg/litre. The study was performed under GLP and meets all validity criteria. Only nominal test concentrations are presented which limits the use of this data when performing a riskassessment when looking at the bio-available fraction
- Executive summary:
The toxicity of pentaethylenehexamine (PEHA) to exponentially growing S. capricornutumhas been assessed over an exposure period of 72 hours. The algal inhibition test was performed according to the EEC/OECD (201) test guidelines One minor deviation from the protocol of the algal inhibition test was introduced.
- The KH2PO4 and NaCO3 concentrations in the test medium were increased to 160 and 100 mg/litre, respectively, to maintain a constant pH.
- Preliminary experiments to determine the concentration range are performed but not reported according to the guidelines.
In the definitive test the algae were exposed to the following nominal concentrations of 0.125, 0.250, 0.500, 1.00 and 2.00 mg/litre. The EbC50 (0 -72 h) and ErC50 of PEHA for S. capricornutumare 1.7 and 0.7 mg/litre, respectively. The NOEC determined of tetraethylenepentamine is 0.25 mg/litre. All tets concentrations are based on nominal. All validity criteria are met and thus the study is valid.
However the usability of the presented data islimited due to the absence of actual measured concentrations and therefore no information on the bioavailable fraction.
Reference
The extinction of the controls increased by a factor of 48 within 72 hours which met the validity criteria. From the extinctions of potassium dichromate an ErC50 and EbC50 (0 -72 h) were determined of 1.6 and 1.2 mg/litre, respectively.
These values of the reference compound are in accordance with data of an international inter-laboratory test showing
that the test organisms have not changed significantly and that the slight modification of the mineral salts medium does not influence the result. The pH in the algal culture should not deviate more than 1.5 unit during the test. The pH measurements show a limited pH increase. Because pentaethylenehexamine is not readily biodegradable the biodegradation of the test compound within 3 days is impossible. The temperature was 23°C.
Description of key information
EC50 (72 h): 1.7 mg/L (nominal)
NOEC (72 h): 0.25 mg/L (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.7 mg/L
- EC10 or NOEC for freshwater algae:
- 0.25 mg/L
Additional information
The acute toxicity of the substance to aquatic algae was tested in a study set up according to OECD guideline 201 and GLP. The freshwater algae Pseudokirchneriella subcapitata was exposed to nominal substance concentrations of 0.125, 0.250, 0.500, 1.000 and 2.000 mg/L. Analytical dose verification was not performed since the substance was expected to be stable under the tested conditions.
Based on the algal growth rate an EC50 (72 h) of 1.7 mg/L (nominal) was determined. The NOEC (72 h) was 0.25 mg/L for algal growth and biomass respectively.
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