Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-999-8 | CAS number: -
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well-documented, peer-reviewed publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Risk assessment of linear alkylbenzene sulphonates, LAS, in agricultural soil revisited: Robust chronic toxicity tests for Olsomia candida (Collembola), Aporrectodea caligninosa (Oligochaeta), and Enchytraeus (Enchytraeidae)
- Author:
- Krogh, PH, Lopez, CV, Cassani, G, Jensen, J, Holmstrup, M, Schraepen, N, Jorgensen, E, Gavor, Z, Temara, A
- Year:
- 2 007
- Bibliographic source:
- Chemosphere 69, 872-879.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Similar to ISO (1998) and ISO 16387 (2004).
- GLP compliance:
- no
Test material
- Reference substance name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- EC Number:
- 270-115-0
- EC Name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- Cas Number:
- 68411-30-3
- IUPAC Name:
- sodium 4-undecylbenzenesulfonate
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Initial concentrations were measured.
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- LABS Na was mixed in demineralized water, and then added to dry Askov soil. Fresh sludge (approx. 200 g) was freeze-dried. This was crushed and sieved through a 2 mm sieve. A methanol solution of LAS was then added to the sludge.
Test organisms
- Test organisms (species):
- other: A. caliginosa, E. crypticus
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: surrounding of National Environmental Research Institute, Silkeborg, Denmark
ACCLIMATION
- Acclimation period: 7 days
TEST ORGANISM
- Source: permanent laboratory cultures
Study design
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 16 degrees C
- Details on test conditions:
- A. caliginosa
TEST SYSTEM
- Test container (material, size): plastic pots
- Amount of soil or substrate: 1 kg soil and 80 ml test solution
- No. of organisms per container (treatment): 4
- No. of replicates per treatment group: 6
- No. of replicates per control: 2
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 76
- % silt: 12
- % clay: 11
- Soil classification system: sandy loam
- Organic carbon (%):1.3
- CEC: 13.4
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Cocoons were collected by sieving with 1 mm mesh after 28 days.
E. crypticus
- Amount of soil or substrate: 20 g soil and 3 ml test solution
- No. of organisms per container (treatment): 3
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 76
- % silt: 12
- % clay: 11
- Soil classification system: sandy loam
- Organic carbon (%):1.3
- CEC: 13.4 - Nominal and measured concentrations:
- A. caliginosa: 25-150 mg/kg soil (nominal)
E. crypticus 0, 10, 40, 60, 80, 100, 120 mg/kg soil (nominal)
0.65, 7.1, 26.3, 45.5, 60.3, 92.4, 102.7 mg/kg soil (measured)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 46 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Remarks on result:
- other: A. caliginosa
- Dose descriptor:
- EC10
- Effect conc.:
- >= 120 mg/kg soil dw
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Remarks on result:
- other: E. crypticus
- Details on results:
- No toxicity to E. crypticus was seen at any dose level, including the highest dose level of 120 mg/kg soil. A dose-related inhibition of cocoon production was seen in A. caliginosa at the highest concentration of 150 mg/kg soil.
Applicant's summary and conclusion
- Conclusions:
- The EC10 for A. caliginosa was 46 mg/kg soil. The EC10 for E. crypticus was >=120 mg/kg soil.
- Executive summary:
Groups of A. caliginosa were exposed to soil containing 25 -150 mg/kg soil of LABS Na for 28 days. Groups of E. crypticus were exposed to concentrations of 0, 10, 40, 60, 80, 100, or 120 mg/kg soil (nominal). EC10 values based on reproduction were 46 mg/kg soil for A. caliginosa, and >= 120 mg/kg soil for E. crypticus.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
