Fir, Abies balsamea, ext.

Melting point / freezing point

Administrative data

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29-31 January 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study has been conducted according to EFFA/EFEO guidance on Natural Complex Substances. The substance is adequately characterised. Therefore full validation applies.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

no guideline required

Principles of method if other than guideline:

The test item is a mixture of constituents and transition from liquid to solid will not be sharp. The Guidance on information requirements and chemical safety assessment Chapter R.7a: Endpoint specific guidance from ECHA (2016) mentioned that "Melting points are not normally carried out for mixtures". In addition, according to column 2, point 7.2 of Annex VII of REACH regulation, "the study does not need to be conducted below a lower limit of -20°C".

GLP compliance:

no

Type of method:

other: visual observation after equilibrium at -20 °C.

Test material

Test material information

Constituent 1

Reference substance name:

Fir, Abies balsamea, ext.

EC Number:

285-364-0

EC Name:

Fir, Abies balsamea, ext.

Cas Number:

85085-34-3

IUPAC Name:

Fir Balsam Oil

Test material form:

liquid

Details on test material:

Name of test material as cited in study report: Fir Needle Oil Canadian (Fir, Abies Balsamea, ext)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Botanical source and Lot# 67919
- Expiration date of the lot/batch: 13 septembre 2018
- Purity test date: UVCB, considered 100% pure

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature (15-25 °C, ≤ 70 RH%), protected from light, avoid contact with iron.

Results and discussion

Melting / freezing point

Melting / freezing pt.:

<= -20 °C

Remarks on result:

other: See pictures attached

Any other information on results incl. tables

RESULTS:

Temperature	+20 °C	-20 °C for 48 h
State of sample 1	Clear mobile liquid	Clear mobile liquid

After a storage time in a freezer at -20 deg C for about 48hr, the test substance was still a clear mobile liquid. Therefore, its melting point is less than -20 deg C and no other test is necessary as per REACH annex VII, column 2, point 7.2.

Applicant's summary and conclusion

Conclusions:

The sample remained liquid at -20 °C therefore the freezing point of the test substance was considered to be < -20 °C.
No further testing is required.

Executive summary:

The physical state of the test substance was observed after 48hr equilibration at -20 °C, according to a method recommended by EFFA/EFEO for NCSs. The sample remained liquid at -20 °C therefore the freezing point of the test substance is < -20 °C (< 253.15 K). No further testing is required.