Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 April to 28 April 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study report is conducted to OECD and EEC guidelines and contains a statement of GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

84/449/EEC

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

EC Number:

403-360-0

EC Name:

Bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Cas Number:

42405-40-3

Molecular formula:

C30H42O6Zn

IUPAC Name:

bis(3,5-bis(1,1-dimethylethyl)-2-hydroxybenzoato-O1,O2)zinc

Details on test material:

- Physical state: white powder
- Analytical purity: not known
- Impurities (identity and concentrations): not known
- Composition of test material, percentage of components: not known
- Lot/batch No.: not known
- Expiration date of the lot/batch: not known
- Storage condition of test material: in a glass container at room temperature in the dark
- Other:

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 515 to 517.2K
- Boiling point: >538K
- Vapour pressure: <0.00001Pa
- Water solubility (under test conditions): 187 mg/l
- Henry's law constant: Not reported
- log Pow: 2.32 at 18C
- pKa: Not reported
- Stability in water: stable in water
- Stability in light: stable in light
- pH dependance on stability: Not reported


OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: 15% biodegradation after 28 days
- Other:

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0.025 - 0.25 μg/mlof test substance in a methanol/ distilled water (50:50) dilution solution.
- Sampling method: 10ml sample made upto volume with methanol/ distilled water (50:50)

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
2.0 g of test material were stirred into 5L of de-ionised water overnight at room temperature. The saturated solution was then filtered through glass fibre paper and diluted to give the anticipated test concentrations of 0.10 to 18.0 mg/l.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: Laboratory culture originating from a strain supplied by IRCHA, France
- Age at study initiation (mean and range, SD): young daphnids produced overnight before the test.
- Valve height at study initiation, for shell deposition study (mean and range, SD): Not reported
- Peripheral shell growth removed prior to test initiation: Not reported
- Method of breeding: Not reported
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Not reported

Test conditions

Hardness:

Total Hardness ≈ 350 mg/l as calcium carbonate. Although slightly higher than the recommended range for this test, it is not considered to have had any significant effects on the results of the test.

Test temperature:

21 ±1C

pH:

pH = 8

Dissolved oxygen:

Mean D.O.: 94.58%

Salinity:

Sodium content: 43.8 mg/l

Nominal and measured concentrations:

Nominal test concentrations: 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6, 10 and 18 mg/l
Measured test concentrations: 0.077, 0.50, 0.45, 0.79, 1.6, 2.8, 4.4, 7.5 and 14 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass Jars
- Type (delete if not applicable): not reported
- Material, size, headspace, fill volume: 200ml test solution
- Aeration: None
- No. of organisms per vessel: 20 animals per test vessel
- No. of vessels per concentration (replicates): two
- No. of vessels per control (replicates): two
- Biomass loading rate: 20 ml test solution per organism



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised laboratory water
- Total organic carbon: mean 4.52 mg/l
- Particulate matter: No information
- Metals: No information
- Pesticides: No information
- Chlorine: mean 0.09 mg/l
- Alkalinity: mean 215.22 mg/l (calcium carbonate)
- Ca/mg ratio: mean Ca: Mg ratio = 16:1
- Conductivity: mean 905.31
- Culture medium different from test medium: No information
- Intervals of water quality measurement: 3 monthly intervals.


OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment
- Photoperiod: 16 h light : 8 h dark
- Light intensity: no information



EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 24 and 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.73 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

other: immobilisation

Remarks on result:

other: 95% CL: 0.59 - 0.9 mg/l

Details on results:

- Behavioural abnormalities: None
- Observations on body length and weight: N/A
- Other biological observations: None reported
- Mortality of control: N/A
- Other adverse effects control: None reported
- Abnormal responses: None reported
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None reported
- Effect concentrations exceeding solubility of substance in test medium: None reported

Results with reference substance (positive control):

No positive control data are included

Reported statistics and error estimates:

None reported

Any other information on results incl. tables

Time (h)	EC 50 (mg/l)	95% confidence limits (mg/l)
24	9.0	6.3 - 13
48	0.73	0.59 - 0.90

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of the test substance to Daphnia Magna is 0.73 mg/l.

Executive summary:

The acute toxcity to Daphnia magna study was conducted with Daphnia magna under static conditions and with analysis provided by HPLC with a UV-visible detector. Conducted over a 48 -hour exposure period, the test substance achieved an EC₅₀of 0.73 mg/l. Based on these test results, the susbstance is proposed to be classified as very toxic to aquatic species.