6-nonyl-1,3,5-triazine-2,4-diamine

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no information available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

initial weight variation in male animals exceeded 20% of the mean weight

GLP compliance:

no

Remarks:

study performed before implementation of GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6-nonyl-1,3,5-triazine-2,4-diamine

EC Number:

227-645-2

EC Name:

6-nonyl-1,3,5-triazine-2,4-diamine

Cas Number:

5921-65-3

Molecular formula:

C12H23N5

IUPAC Name:

6-nonyl-1,3,5-triazine-2,4-diamine

Test material form:

solid: bulk

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: young adults
- Weight at study initiation: males 169-286 g and females 163-194 g
- Fasting period before study: overnight
- Housing: in groups of five in screenbottomed stainless steel cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25°C

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

DMSO

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 50% w/v
- Amount of vehicle (if gavage): 8 mL/kg bw
- Justification for choice of vehicle: preliminary observations

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg bw

Doses:

10 g/kg bw

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: several times within first 24 hours, after that daily; no body weight recording
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

act. ingr.

Remarks on result:

other:

Remarks:

1/5 males and 1/5 females died

Mortality:

One male and one female died on the second and third day after test substance treatment, respectively

Clinical signs:

other: Within a few hours after treatment the rats showed sluggishness and several rats lost consciousness for a few hours. One male and one female which died later showed encrustrations round eyes and nostrils. The survivors recovered within 24 hours and look

Gross pathology:

No abnormalities were observed at necropsy.

Any other information on results incl. tables

None of the rats treated with DMSO showed reaction upon treatment.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The oral LD50 of Caprinoguanamine in rats was > 10000 mg/kg bw.

Executive summary:

In an acute oral toxicity study similar to OECD guideline 401, 5 male and 5 female, fasted, young Wistar strain rats were given a single oral dose of Caprinoguanamine in DMSO at a limit dose of 10000 mg/kg bw and observed for 14 days. 5 male and 5 female rats were treated with an equal amount of DMSO (8 mL/kg bw).

Within a few hours after treatment the rats showed sluggishness and several rats lost consciousness for a few hours. One male and one female showed encrustations round eyes and nostrils. They died on the second and third day after test substance treatment, respectively. The survivors recovered within 24 hours and looked quite healthy at the end of the observation period. None of the rats treated with DMSO showed any reaction upon treatment. No abnormalities were observed at necropsy.

Oral LD50 (rat, females/males) > 10000  mg/kg bw