Decanedioic acid, compound with ethanolamine / Decanedioic acid, compound with 2-aminoethanol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 30, 2017 - August 3, 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

Organisation for Economic Co-operation and Development (OECD) Guideline 301D, “301D
Closed Bottle Test” (OECD, 1992)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Sebacic acid, compound with 2-aminoethanol (1:2)

Molecular formula:

C10H18O4.2(C2H7NO)

IUPAC Name:

Sebacic acid, compound with 2-aminoethanol (1:2)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

An inoculum containing an active microbial population was prepared as follows. Six days prior to
test initiation, a sample of secondary effluent was collected from the Guelph Wastewater
Treatment Plant (Guelph, Ontario). The secondary effluent was continuously aerated during
transport to, and storage at AquaTox, and then pre-conditioned for testing. During preconditioning,
the secondary effluent was maintained at test temperature (22 ± 2 °C) prior to test
initiation. The secondary effluent was settled for 30 minutes prior to the clear supernatant being
decanted for use as the inoculum.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

3.35 mg/L

Based on:

COD

Remarks:

4 mg/L COD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

D.O. measurement

Details on study design:

Sampling done on Days 0, 4, 7, 11, 14, 21 & 28

For each sampling day, the following bottle plan was used:

Non-incoculated control: 1 bottle
inoculated control: 2 bottles
test series: 2 bottles
reference series (procedural control): 2 bottles
inhibition control (toxicity control): 2 bottles

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

84

Sampling time:

28 d

Remarks on result:

other:

Remarks:

readily biodegradable

Details on results:

The test item achieved 84.0% degradation over the 28-day exposure. Furthermore, based on
visual inspection of the biodegradation curve, the test item achieved approximately 75%
degradation within 10 days after achieving 10% biodegradation (i.e., the 10 day window).
According to the strict requirements of OECD 301D, the test item can be considered to be
readily biodegradable.

BOD5 / COD results

Results with reference substance:

The reference substance (KHP) achieved 85% degradation in 14 days. The toxicity control achieved 84.4% degradation in 14 days.

Any other information on results incl. tables

Test day	0	4	7	11	14	21	28
Degradation (%)	0.0	55.0	56.2	76.2	85.0	87.5	84.0

The test item achieved 84% degradation in 28 days. Based on the visual inspection of the biodegradaiton surve, the test item achieved approximately 75% degradation 10 days after achieving 10% degradation .

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item, Sebacic acid, compound with 2-aminoethanol achieved 84% degradation in 28 days. All validity criteria were met and the test item can be considered to be readily biodegradable.

Executive summary:

AquaTox Testing & Consulting Inc. (AquaTox) was retained by the Study Sponsor, Afton

Chemical Corporation, to conduct a study to determine the biodegradability of the test item,

Sebacic Acid MEA Salt (Lot/Batch No. LAB 4408; AquaTox test item number 50508). The study

was conducted in accordance with AquaTox Study Plan 162775728-301 entitled “Sebacic Acid

MEA Salt: Ready Biodegradability Closed Bottle Test (OECD 301D)” (Appendix A) and

AquaTox Standard Operating Procedure (SOP) # 392 (AquaTox, 2016a), which are based on

Organisation for Economic Co-operation and Development (OECD) Guideline 301D, “301D

Closed Bottle Test” (OECD, 1992). The study was conducted in support of global registration.

All components of the study were conducted according to OECD Principles of Good Laboratory

Practice (GLP) (OECD, 1998) with the exception noted in the GLP Compliance Statement.

Testing was initiated on July 6, 2017 and was completed on August 3, 2017. The concentration

of the test item in the mineral medium was 3.35 mg/L. The corresponding Chemical Oxygen

Demand (COD) was 4.0 mg/L. The quality of the test system was confirmed through the use of

a reference substance (potassium hydrogen phthalate, Fisher Scientific, Lot # 034159) and all

of the criteria for test validity were met.

The test item achieved 84.0% degradation over the 28-day exposure. Furthermore, based on

visual inspection of the biodegradation curve, the test item achieved approximately 75%

degradation within 10 days after achieving 10% biodegradation (i.e., the 10 day window).

According to the strict requirements of OECD 301D, the test item can be considered to be

readily biodegradable.

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