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Diss Factsheets

Toxicological information

Skin irritation / corrosion

Currently viewing:

Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: The Federal Hazardous substances Act (code of Federal Regulations, Title 16, Chapter II, 1976)
Principles of method if other than guideline:
- Principle of test:0.5 gram of the test substance was applier on the trunk of 6 rabbit for 24 hours on intact or abraded skin.

GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzyltriphenylphosphonium chloride
EC Number:
214-154-3
EC Name:
Benzyltriphenylphosphonium chloride
Cas Number:
1100-88-5
Molecular formula:
C25H22P.Cl
IUPAC Name:
benzyltriphenylphosphonium chloride
Test material form:
solid: particulate/powder
Details on test material:
Argentimetric title: 100%
weight loose: 0.2%

Test animals

Species:
rabbit
Strain:
other: albino
Details on test animals or test system and environmental conditions:
No data

Test system

Type of coverage:
not specified
Preparation of test site:
clipped
Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 g of undiluted sample which was moistened with physiological saline to form a paste was applied under a one inch-square surgical gauze patch.
Duration of treatment / exposure:
24 hours
Observation period:
72 hours
Number of animals:
6 albino rabbits
Details on study design:
TEST SITE
- Area of exposure: Two layers thick (an intact skin area and an abraded skin area). The application sites were prepared by clipping the hair from the saddle area of the rabbits.The abraded areas were prepared by making minor epidermal incisions with a hypodermic needle. The abrasions, were sufficiently deep to penetrate the epidermis but not to induce bleeding.
- % coverage: no data
- Type of wrap if used: the trunk was wrapped with rubbe. An outer layer of gauze and tape was placed around the trunk of the animals.

OBSERVATION TIME POINTS
24 and 72 hours

SCORING SYSTEM:
- Method of calculation: similar to Draize score.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #4
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #5
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #6
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #1
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #2
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #3
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #4
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #5
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
animal #6
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #1
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #2
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #3
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #4
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #5
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #6
Time point:
24 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
erythema score
Basis:
mean
Time point:
48 h
Remarks on result:
not measured/tested
Irritation parameter:
edema score
Basis:
mean
Time point:
48 h
Remarks on result:
not measured/tested
Irritation parameter:
edema score
Basis:
animal #1
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #2
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #3
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #4
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #5
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
edema score
Basis:
animal #6
Time point:
72 h
Score:
0
Max. score:
4
Reversibility:
other: not applicable
Remarks on result:
no indication of irritation
Irritation parameter:
primary dermal irritation index (PDII)
Basis:
mean
Time point:
other: 24/72 hours
Score:
0
Reversibility:
other: Not applicable as no effect
Irritant / corrosive response data:
No irritative or corrosive effects were noted at either observation period. The Primary irritation Index was found to be 0.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Under the conditions of this test, no irritation was observed on skin rabbit after a 24-hour exposure period.
Executive summary:

This study was conducted to evaluate the primary skin irritative potential of Benzyltriphenylphosphonium chloride. Five-tenths of the undiluted sample was moistened with physiological saline to form a paste was applied under a one inch-square surgical gauze patch, two layers thick, to an intact skin area and an abraded skin area on each of six albino rabbits. The application sites were prepared by clipping the hair from the saddle area of the rabbits. The abraded areas were prepared by making minor epidermal incisions with a hypodermic needle. The abrasions, were sufficiently deep to penetrate the epidermis but not to induce bleeding. Each patch was held in place with two strips of one-inch adhesive tape. After application of the patches, the trunk of each rabbit was wrapped with rubbe and secure with staples. An outer layer of gauze and tape was placed around the trunk of the animals. The animals were restrained in Newmann harnesses for 24 hours. No irritative or corrosive effects were noticed at either observation period. The Primary Irritative Index was found to be 0. Based on results from this test, no classification is required for Benzyltriphenylphosphonium Chloride.