Registration Dossier
Registration Dossier
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EC number: 221-698-5 | CAS number: 3195-78-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The ready biodegradability of the test substance was examined according to the OECD 301 B (CO2 evolution, modified Sturm test). The test substance was degraded 80 - 90 % CO2/ThCO2 at the end of the ten-day window and 90 – 100 % DOC at the end of exposure. The test substance was found to be readily biodegradable under the test conditions and the required pass level for ready biodegradability within a ten days window was reached.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The ready biodegradability of the test substance was examined according to the OECD 301 B (CO2 evolution, modified Sturm test). The biodegradation of the test substance was followed by exposing it to the active sludge of a domestic waste water treatment plant from Mannheim (Germany). The inoculum was aerated for about 24 hours at 22 +/- 2 °C. The active sludge concentration was 30 mg/L (dry weight). The test concentration was 20 mg/L TOC nominal, equivalent to approx. 33 mg/L test substance. As a reference compound (procedure control), aniline was tested simultaneously under the same conditions. No inhibitory effect on the microorganisms was observed. The degree of the biodegradation in the inhibition control after 14 days was determined to be 68 % CO2/ThCO2. In the procedure control, the reference compound aniline was readily biodegraded by an average of 83 % CO2/ThCO2 after 14 days of exposure. The validity criteria were met. The test substance was degraded 80 - 90 % (mean value) concerning CO2/ThCO2 at the end of the ten-day window and at the end of exposure and 90 – 100 % concerning DOC at the end of exposure. The test substance was found to be readily biodegradable under the test conditions and the required pass level for ready biodegradability within a ten days window was also reached.
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