Registration Dossier
Registration Dossier
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EC number: 219-599-7 | CAS number: 2475-33-4
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- March 28, 1976
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- The reliability assigned to this study is 4 as the composition of the active ingredient in the test item is low.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Vat Brown 001
- IUPAC Name:
- Vat Brown 001
- Test material form:
- solid: particulate/powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Tif RAlf
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CIBA-GEIGY LIMITED
- Weight at study initiation: from 105 to 115 grams
- Fasting period before study: animals fasted overnight before treatment
- Housing: in groups of 5 in Macrolon cages
- Diet (e.g. ad libitum): standard diet of pellets - No. 890, Nafag Gossau SG ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: for a minimum of 5 days before treatment
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1 °C
- Humidity (%): 55 ± 5 %
- Photoperiod (hrs dark / hrs light): 14 hours light cycle day
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: distilled water
- Doses:
- 1000, 3000, 10000, 15000 mg/kg bw
- No. of animals per sex per dose:
- 5 per sex per dose
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 15 days
- Other examinations performed: clinical signs, body weight,organ
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 15 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no deaths occured
- Clinical signs:
- At 10000 mg/kg bw: reduction in spontaneous motility, ataxia, muscular hypotonia. Symptoms lasting >6 hours. After 24 hours no symptoms.
At 15000 mg/kg bw: ditto, diarrhea.
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP criteria not met
- Conclusions:
- LD50 > 15000 mg/kg bw
- Executive summary:
The acute oral LD50 of the test substance in rats of both sexes observed over a period of 15 days is >15000 mg/kg bw.
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