2-methoxybenzyl alcohol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed publication

Justification for type of information:

Data is from publication.

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity studies of p-substituted benzyl derivatives in the Ames Salmonella plate-incorporation assay

Author:

James C. Ball, Susan Foxall-VanAken and Trescott E. Jensen

Year:

1984

Bibliographic source:

Mutation Research, 138 (1984) 145-151

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mention below

Principles of method if other than guideline:

Gene mutation study was performed to evaluate the mutagenic nature of the test compound Benzyl alcohol

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl alcohol

EC Number:

202-859-9

EC Name:

Benzyl alcohol

Cas Number:

100-51-6

Molecular formula:

C7H8O

IUPAC Name:

Benzenemethanol

Details on test material:

- Name of test material: Benzyl alcohol
- Molecular formula: C7H8O
- Molecular weight: 108.1392 g/mol
- Substance type: Organic
- Physical state: No data available
- Purity: No data available
- Impurities (identity and concentrations): No data available

Specific details on test material used for the study:

- Name of test material: Benzyl alcohol
- Molecular formula: C7H8O
- Molecular weight: 108.1392 g/mol
- Substance type: Organic

Method

Target gene:

Histidine

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA100 and TA98

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

not specified

Metabolic activation system:

No data

Test concentrations with justification for top dose:

100, 250, 500, 1000 µg/plate

Vehicle / solvent:

No data available

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

methylmethanesulfonate

Details on test system and experimental conditions:

Details on test system and conditions
METHOD OF APPLICATION: in agar (plate incorporation)

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: Duplicate

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available

Evaluation criteria:

A compound was considered to be mutagenic if the number of revertants/plate exceeded the 99.9% confidence limit and a concentration-dependent increase in the mutagenicity was observed. The 99.9% confidence limit was 99 revertants above spontaneous revertant levels for TA100 (spontaneous revertants = 196 ± 29 revertants/plate) and 40 revertants above spontaneous revertants for TA98 (spontaneous revertants = 40 ± 12 revertants/plate).

Statistics:

The 99.9% confidence limit was calculated by multiplying the standard deviation from 27 independent observations (duplicate determinations) by the appropriate t value of the Student's t distribution (one-sided) requiring 99.9% confidence (Skopek et al., 1978).

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA100 and TA98

Metabolic activation:

not specified

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data available

Remarks on result:

other: No mutagenic effect were observed.

Applicant's summary and conclusion

Conclusions:

Benzyl alcohol (100-51-6) was failed to induce an increase in the number of revertants/plate and hence is not likely to be mutagenic in vitro.

Executive summary:

Gene mutation study was performed to evaluate the mutagenic nature of the test compound Benzyl alcohol. Ames Salmonella plate-incorporation assay was performed using Salmonella typhimurium strain TA100 and TA98. The test concentration were 0,100, 250, 500, 1000 µg/plateThe test compound benzyl alcohol failed to induce an increase in the number of revertants/plate and hence is not likely to be mutagenic in vitro.