Sodium prop-2-enesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF

HOUSING CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: ca. 5 µE/m²s at a wavelength of 400-750 nm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.78 mmol/L

Test temperature:

ca. 21 °C

pH:

7.9 - 8.2

Dissolved oxygen:

Study initiation: 9.00 - 9.25 mg/L
Study end: 8.51 - 8.76 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 62.5, 125, 250, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, fill volume: centrifuge tubes with flat bottom, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water (conductivity < 0.05 µS/cm) aerated with oil-free air for 24 h
- Alkalinity: 0.92 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 660 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content after 0 and 48 hours

OTHER TEST CONDITIONS
- Light intensity: diffuse light

EFFECT PARAMETERS MEASURED: mobility after 0, 3, 6, 24, 48 hours

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Details on results:

- Mortality of control: none

Description of key information

With high probability sodium prop-2-enesulphonate is acutely not harmful to aquatic invertebrates.

Additional information

The acute toxicity of sodium prop-2-enesulphonate was studied with the water flea Daphnia magna, in accordance to EU Method C.2 [BASF AG, 1989]. Up to the highest test concentration of 500 mg/L sodium prop-2 -enesulphonate no effect observed.