Registration Dossier
Registration Dossier
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EC number: 609-330-5 | CAS number: 370865-89-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-ethyl-1-methyl-1H-Imidazolium salt with N-cyanocyanamide (1:1)
- EC Number:
- 609-330-5
- Cas Number:
- 370865-89-7
- Molecular formula:
- C6 H11 N2 .C2 N3
- IUPAC Name:
- 3-ethyl-1-methyl-1H-Imidazolium salt with N-cyanocyanamide (1:1)
Constituent 1
- Specific details on test material used for the study:
- - Name of the test material (as cited in study report): EMIM Dicyanamid
- Purity/composition: 97.4 g/100 g
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- liver S9 mix from induced rats
- Test concentrations with justification for top dose:
- 33 µg - 5200 µg/plate (dose levels for Standard Plate Test and Preincubation Test)
- Vehicle / solvent:
- Due to the good solubility of the test substance in ultrapure water, ultrapure water was used as vehicle.
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- other: 2-aminoanthracene; N-methyl-N'-nitro-N-nitrosoguanidine, 4-nitro-o-phenylenediamine
- Details on test system and experimental conditions:
- Standard Plate Test and Preincubation Test
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: No bacteriotoxic effect was observed under all test conditions.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No precipitation of the test substance was found with and without S9 mix.
Applicant's summary and conclusion
- Conclusions:
- GHS criteria not met
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