2-allylphenol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Algal growth inhibition of Chlorella pyrenoidosa by polar narcotic pollutants: toxic cell concentrations and QSAR modelling

Author:

Enaut Urrestarazu Ramos, Wouter H.J. Vaes, Philipp Mayer, Joop L.M. Hermens

Year:

1999

Bibliographic source:

Aquat Toxicol 46:1-10

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Effects on the population growth of algae was tested according to the OECD guidelines for testing chemicals. Algae were exposed for 72h, in triplicate, to five concentration levels and one control. Concentration levels were prepared with a dilution factor of 2.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-allylphenol

EC Number:

217-119-0

EC Name:

2-allylphenol

Cas Number:

1745-81-9

Molecular formula:

C9H10O

IUPAC Name:

2-(prop-2-en-1-yl)phenol

Details on test material:

- Name of test material (as cited in study report): 2-allylphenol
- Analytical purity: 98%
- Other: Supplier: Aldrich-Chemie, Steinheim, Germany

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

Stock solutions were prepared directly in the culture medium, and were stirred for at least 24 h. The stock solutions were used to prepare
fresh test solutions by dilution with medium. Tests were performed in 40 ml vials containing 20 ml solution, to which 0.5 ml inoculum (approximately
2e+06 cells/ml) was added.

Test organisms

Test organisms (species):

Chlorella pyrenoidosa

Details on test organisms:

The algae were cultured in Miller’s solution using a 100 ml bottle, which was renewed (1:5) at least once a week. The algal cultures were incubated on top of a series of TL tubes, and were shaken by a microplate shaker at 300 rpm.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

approximately 22°C

pH:

approximately 7.4

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

5.3 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

not specified

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

4.9 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

not specified

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

2.7 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

not specified