Octane-1-thiol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980-12-19 to 1981-01-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study was classified as reliable with restrictions because all tests conducted were in accordance with GLP and the test protocol followed is similar to OECD guideline 405.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Octane-1-thiol (CAS # 111-88-6)

IUPAC Name:

Octane-1-thiol (CAS # 111-88-6)

Details on test material:

- Name of test material (as cited in study report): n-octyl mercaptan (Octane-1-thiol)
- Substance type: Heavy Mercaptan
- Physical state: Liquid

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: University of Utah Vivarium
- Housing: Rabbits were housed individually in wire cages with stainless steel pans
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 day


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 +/- 3
- Humidity (%): 30 to 60
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12 / 12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 milligrams or 0.1 mL

Duration of treatment / exposure:

exposure lasted 4 seconds

Observation period (in vivo):

7 days

Number of animals or in vitro replicates:

6 New Zealand white albino rabbits

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no
- Time after start of exposure: 4 seconds


SCORING SYSTEM: Draize et al. 1944


TOOL USED TO ASSESS SCORE: hand-held ophthalmoscope

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 24, 48, and 72 hours

Score:

ca. 0.4

Max. score:

110

Reversibility:

fully reversible within: 7 days

Irritant / corrosive response data:

Octane-1-thiol demonstrated irritation in 3 out of 6 rabbits using the primary eye irritation test (unwashed). Clinical signs of irritation observed were slight redness at the 1 hour observation, with secreted discharge observed in one animal at day 4. All animals had recovered by day 7.

Other effects:

All animals were weighed on the day of arrival, the day of dosing and day 7. The combined mean weight gain from day of dosing to day 7 was approximately 254 grams.

Any other information on results incl. tables

	Score								Number of Animals with Eye Response
							Total
Animal Number	550	551	552	553	554	555	Ave.	Total
Observation, Prescreen	0	0	0	0	0	0	0	0	0
1 hour	2	2	0	0	2	0	1.0	0-2	3
24 hour	0	2	0	0	0	2	0.7	0-2	2
48 hour	0	2	0	0	0	0	0.3	0-2	1
72 hour	0	2	0	0	0	0	0.3	0-2	1
4 day	0	0	0	0	0	2	0.3	0-2	1
7 day	0	0	0	0	0	0	0	0	0
Combined Average	0.3	1.3	0	0	0.3	0.7	0.4	0-2

Applicant's summary and conclusion

Interpretation of results:

slightly irritating

Remarks:

Migrated information (Not classified) Criteria used for interpretation of results: EU

Conclusions:

Octane-1-thiol demonstrated irritation in 4 out of 6 rabbits using the Primary Eye irritation test (unwashed). The mean Draize score of 0.4 was tabulated from the 6 rabbits including 6 observation periods.

Executive summary:

In a primary eye irritation study, 0.1 mL of octane-1-thiol was instilled into the conjunctival sac of the right eye of 6 young adult New Zealand white rabbits for 4 seconds while the untreated left eye served as control. Animals then were observed for 7 days. Irritation was scored by the method of the Draize Primary Eye Irritation Test (unwashed).

 

Octane-1-thiol demonstrated irritation in 4 out of 6 rabbits, with a mean Draize score of 0.4.  In this study, octane-1-thiol is slightly irritating to the eye because most of the effects observed were reversible in the first 24 hours post exposure, and all were reversible 7 days after exposure.

 

This study received a Klimisch score of 1 and was classified as reliable with restrictions because all tests conducted were in accordance with GLP and the test protocol followed is similar to OECD guideline 405.