1,2-dichloro-3-nitrobenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

adopted in 1981

Deviations:

yes

Remarks:

8 d observation period

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,2-dichloro-3-nitrobenzene

EC Number:

221-717-7

EC Name:

1,2-dichloro-3-nitrobenzene

Cas Number:

3209-22-1

Molecular formula:

C6H3Cl2NO2

IUPAC Name:

1,2-dichloro-3-nitrobenzene

Details on test material:

- Analytical purity: No data

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Lippische Versuchstierzucht, Hagemann GmbH & Co. KG, Extertal, Germany
- Weight at study initiation: 2.7 kg
- Housing: single
- Diet (e.g. ad libitum): mümmel z (ssniff, Soest, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: clipped skin

Vehicle:

other: oil (not further specified)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 500 mg

Duration of treatment / exposure:

4 h

Observation period:

1, 24, 48 and 72 h, and 8 days

Number of animals:

3

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at any time point

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

4

Reversibility:

other: score = 0 at any time point

Results 3

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 h

Score:

0

Results 4

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 48 h

Score:

0

Results 5

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 72 h

Score:

0

Results 6

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 h

Score:

0

Results 7

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 48 h

Score:

0

Results 8

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 72 h

Score:

0

Any other information on results incl. tables

Erythema/edema scores

	Time point after patch removal	Animal No.			Sum	Average
		1	2	3
Erythema score	1 h	0	0	0
	24 h	0	0	0	0	0
	48 h	0	0	0	0	0
	72 h	0	0	0	0	0
	8 days	0	0	0
Edema score	1 h	0	0	0
	24 h	0	0	0	0	0
	48 h	0	0	0	0	0
	72 h	0	0	0	0	0
	8 days	0	0	0

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Executive summary:

Schreiber G (1982)

The skin irritation potential of 1,2-dichloro-3-nitrobenzene was evaluated according to the OECD guideline 404 (adopted: 12 May 1981). 0.5 g 1,2 -dichloro-3 -nitrobenzene ground in oil was applied to the clipped left flank of each of 3 New Zealand White rabbits for 4 h under semi-occlusive conditions. Thereafter patches and test material were removed. The test sites were examined and scored for erythema and edema using the Draize method 1, 24, 48 and 72 h as well as 8 days after exposure. The mean erythema and edema scores over 24, 48 and 72 h were 0.0 for both end points in all animals. No signs for skin irritation were observed throughout the test and observation period.