[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015/10/13 - 2015/12/18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The test was performed following a suitable standard test guideline with GLP. The validity criteria were fulfilled.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

EC Number:

244-096-4

EC Name:

[2-[(2-methyl-1-oxoallyl)oxy]ethyl] hydrogen succinate

Cas Number:

20882-04-6

Molecular formula:

C10H14O6

IUPAC Name:

4-{2-[(2-methylprop-2-enoyl)oxy]ethoxy}-4-oxobutanoic acid

Test material form:

other: liquid

Details on test material:

Company internal substance number SAT 150014, Batch3903/64
Colorless liquid at room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Aerobic activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage plant Rossdorf, Germany.

Duration of test (contact time):

>= 28 d

Initial test substance concentration

Initial conc.:

>= 102 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The test item was exposed to aerobic activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions
as the test item, and functioned as a procedure
control.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

>= 80

Sampling time:

28 d

Details on results:

The mean biodegradation of 10% of SAT 150014 was reached at day 2 (ThODNH4). At the end of the 10-day window at day 12, the degradation of SAT 150014 was 47% (ThODNH4) and therefore the 10 day window criterion was not passed. The mean biodegradation at test end after 28 days was 80% (ThODNH4).
Therefore, SAT 150014 is considered to be readily biodegradable
based on ThODNH4, but failing 10-day window.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

The mean biodegradation of 10% of SAT 150014 was reached at day 2 (ThODNH4). At the end of the 10-day window at day 12, the degradation of SAT 150014 was 47% (ThODNH4) and therefore the 10 day window criterion was not passed. The mean biodegradation at test end after 28 days was 80% (ThODNH4).
Therefore, SAT 150014 is considered to be readily biodegradable
based on ThODNH4, but failing 10-day window.

Executive summary:

The mean biodegradation of 10% of SAT 150014 was reached at day 2 (ThODNH4). At the end of the 10-day window at day 12, the degradation of SAT 150014 was 47% (ThODNH4) and therefore the 10 day window criterion was not passed. The mean biodegradation at test end after 28 days was 80% (ThODNH4). Therefore, SAT 150014 is considered to be readily biodegradable

based on ThODNH4, but failing 10-day window.