A mixture of: 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C16)alkylacetamide; 2,2',2'',2'''-(ethylenedinitrilotetrakis-N,N-di(C18)alkylacetamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 84/449/EEC, C.1 ("Acute toxicity for fish")

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test concentrations were analyzed by determination of DOC in the test water. Test samples were taken at 1 hour and 96 hours after test start.

Vehicle:

yes

Details on test solutions:

The compound was added to the test water separately for each concentration by mixing with 1 g Tween 80 (100 mg/L). Then, aqua bidest was added in small quantities and the solution was transferred into the test water. A solvent control with the same concentration of solubilizer was run in parallel.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Common name: Zebra fish
- Source: West Aquarium GMBH, Bad Lauterberg, FRG
- Length at study initiation: 2.9 cm (2.2 - 3.4)
- Weight at study initiation: 0.25 g (0.17 - 0.37)
- Duration of housing and adaptation: about 1.5 months
- Mortality during the last 2 weeks of housing: about 0.8 %
- Medical treatment: None
- Adaptation to the test water: 7 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

total hardness: 2.5 mmol/L

Test temperature:

22-23 °C

pH:

about 8

Dissolved oxygen:

continuous aeration

Nominal and measured concentrations:

Based on the results of several range finding studies, the test substance was tested at a range of concentrations: 0, 10.0, 21.5, 46.4, and 100 mg/L.

Details on test conditions:

- Photoperiod: 16 hours light and 8 hours darkness
- Volume of water: 10 Liters
- No. of animals per test concentration: 10
- Test vessels: All-glass aquarium (30 cm X 22 cm x 24 cm)
- Test water: Reconstituted freshwater according to DIN 38 412, Part 11
- Aeration: Continuous with oil-free air

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Mortality / Symptoms:
---------------------
No mortality or any symptoms of intoxication were observed at any test concentration.

Concentration control analysis:
-------------------------------
Nominal Conc. DOC [mg/l]
1 h 96 h
-----------------------------------------------------------
10.0 47.5 58.4
21.5 43.9 57.1
46.4 41.6 57.3
100 47.2 55.6

control 1.5 0.6
solvent control 44.1 56.5
-----------------------------------------------------------

The results of the concentration control analysis confirmed the insolubility of the test substance in water even in the presence of a solubilizer.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L