β-methylaziridine-1-propiononitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April to May 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented, scientifically acceptable study report

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Principles of method if other than guideline:

according to BASF-internal standard

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

β-methylaziridine-1-propiononitrile

EC Number:

223-798-4

EC Name:

β-methylaziridine-1-propiononitrile

Cas Number:

4078-19-7

Molecular formula:

C6H10N2

IUPAC Name:

3-(aziridin-1-yl)butanenitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Mean body weight: males 198 g, females 168 g
The animals were offered a standardized animal laboratory diet (Herilan MRH).

Administration / exposure

Type of coverage:

occlusive

Vehicle:

olive oil

Details on dermal exposure:

The test concentrations used were 15, 25, 45 and 50 % (G/G).
Application area: ca. 50 cm2
Application site: back

Duration of exposure:

24 hours

Doses:

100, 200, 400, 681 and 1000 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical and local signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 300 mg/kg bw

Based on:

test mat.

Mortality:

100 mg/kg bw: no deaths after 14 days; 200 mg/kg bw: 3/6 after 14 days (only males died, starting after 7 days); 400 mg/kg bw: 2/6 after 14 days (1 male after 48 h and 1 female after 7 days); 681 and 1000 mg/kg bw: 6/6 after 14 days (starting after 24 h)

Clinical signs:

other: Dyspnea, apathy, excitation, staggering, twitching, spastic gait, flexion convulsions, biting convulsions, diarrhea, anemic paleness, exsiccosis, salivation, poor general state, ruffled fur, breathlessness, clotty eyes, leath deaths.

Gross pathology:

Animals that died: heart: acute dilatation (right); liver: manifold slight brightened; kidneys; isolated yellow-white, radiate striation in the papilla region (papilla necrosis); animals manifold severe emaciated.
Sacrificed animals: kidneys: distinct papilla necrosis.

Other findings:

Local findings:
1000 mg/kg bw: After 24 hours questionable reddening, slight edema.
681 mg/kg bw: After 24 hours questionable reddening, slight edema.
400 mg/kg bw: After 24 hours questionable reddening, slight edema. After 7 days slight scaling (males only), otherwise without findings.
200 mg/kg bw: After 24 hours questionable reddening, slight edema; after 6 hours: slight scaling, very slight up to slight edema; after 14 days without findings.
100 mg/kg bw: After 24 hours questionable reddening, slight edema; after 7 days without findings.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information

Conclusions:

The acute dermal LD 50 was around 300 mg(kg bw for male and female rats.

Executive summary:

The application of the test substance onto the back of female and male rats in doses of 100, 200, 400, 681 and 1000 mg/kg bw. Lead to deaths and different clinical signs in the animals. The acute dermal LD50 was determined to be around 300 mg/kg bw for male and female rats.