5-nitrofurfurylidene di(acetate)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1977

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

(only one strain TA100 tested, without metabolic activation, no positive control included).

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

5-nitrofurfurylidene di(acetate)

EC Number:

202-166-1

EC Name:

5-nitrofurfurylidene di(acetate)

Cas Number:

92-55-7

Molecular formula:

C9H9NO7

IUPAC Name:

(acetyloxy)(5-nitrofuran-2-yl)methyl acetate

Test material form:

solid

Specific details on test material used for the study:

Chemical name: 5-Nitro-2-furanmethandiol diacetate
Source: Aldrich.

Method

Target gene:

Histidine requiring strain

Species / strain

Species / strain / cell type:

S. typhimurium TA 100

Metabolic activation:

without

Test concentrations with justification for top dose:

0.1, 0.5, 1.0, 5.0, 10.0, and 25.0 µg/plate.

Vehicle / solvent:

Dimethyl sulfoxide (Aldrich).

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

no

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 100

Metabolic activation:

without

Genotoxicity:

positive

Remarks:

weak mutagen

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(probably at 25 µg/plate)

Vehicle controls validity:

valid

Any other information on results incl. tables

Dose µg/plate	TA100
0	74	76
0.1	74
0.5	55
1.0	61
5.0	64
10.0	120
25.0	0

The substance appears to be a weak mutagen.

Applicant's summary and conclusion

Conclusions:

The substance appeared to be a weak mutagen in TA100 strain without metabolic activation.

Executive summary:

The mutagenicity of 5 -nitrofurfurylidene diacetate was tested in the Salmonella typhimurium tester strain TA100. The nitrofuran was dissolved in dimethyl sulfoxide (DMSO) and incorporated uniformly in the top agar overlay. Revertant his+ colonies were counted after incubation at 37°C for 48 h. The nitrofuran compound was tested at 0.1, 0.5, 1.0, 5.0, 10.0, and 25.0 µg/plate. The substance appeared to be a weak mutagen in TA100 strain without metabolic activation.