Benzenamine, N-phenyl-, styrenated

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986-10-02 - 1986-10-30 (experimental phase)

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Version / remarks:

The method was modified according to the EC ring test "Assessment of biodegradability of chemicals in water by manometric respirometry (DG X 1 283/82 REV 5)

Deviations:

not specified

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): 3L-Labornanlage (Wupperverband)

Duration of test (contact time):

28 d

Initial conc.:

>= 24.5 - <= 25.9 other: per flask, 250 ml medium per flask

Based on:

test mat.

Remarks:

target: 25 mg

Initial conc.:

100 mg/L

Based on:

test mat.

Remarks:

target concentration

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

SAMPLING
- Sampling frequency: every other day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: mercury

Reference substance:

aniline

Remarks:

target: 100 mg/l

Test performance:

positive control gave appropriate response

Key result

Parameter:

% degradation (O2 consumption)

Value:

9

Sampling time:

28 d

Remarks on result:

other:

Remarks:

not readily biodegradable

Results with reference substance:

positive control gave appropriate response, see table below

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Ready biodegradation was determined acc. OECD 301C with some information lacking. However, given data indicate that the test was properly performed, the positive control gave the appropriate results. Hence, the study is considered to be sufficiently reliable to assess the ready biodegradation of the test item. Biodegradation was determined to be 9% (O2 consumption) after 28 days, hence, the test item is considered to be not readily biodegradable.

Executive summary:

Ready biodegradation of the test item was determined acc. OECD 301C, aniline was the positive control and gave the appropriate results. Biodegradation of the test item was determined to be 9% (O2 consumption) after 28 days, it is not readily biodegradable.

Description of key information

Biodegradation in water, screening test: not readily biodegradable: 9% (O2 consumption) in 28 days (OECD 301C)

Additional information

Ready biodegradation of the test item was determined acc. OECD 301C, aniline was the positive control and gave the appropriate results. Biodegradation of the test item was determined to be 9% (O2 consumption) after 28 days, it is not readily biodegradable.

[Type of water: freshwater]