2-propylvaleronitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-propylvaleronitrile

EC Number:

236-338-2

EC Name:

2-propylvaleronitrile

Cas Number:

13310-75-3

Molecular formula:

C8H15N

IUPAC Name:

2-propylpentanenitrile

Sampling and analysis

Analytical monitoring:

yes

Remarks:

GC-FID

Test organisms

Test organisms (species):

Daphnia magna

Study design

Water media type:

other: reconstituted water (Elendt "M4")

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Visual observation after 48h of the mobility of animals after agitation of the test beaker.

Test conditions

Hardness:

2.5 mmol/L (=250 mg/L) as CaCO3

Test temperature:

20°C (water temperature)

pH:

8.0 to 8.4

Dissolved oxygen:

9.0 to 10.5 mg/L

Nominal and measured concentrations:

Nominal: 6.25, 12.5, 25, 50, 100 mg test item/L
Measured: not specified

Details on test conditions:

Photoperiod: 16h light-8h dark; light intensity: 490 to 950 lux

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

<= 30.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

<= 12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

<= 25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

After 48 hours of exposure, no immobilisation of the test animals was observed in the control and up to and ind including the test item concentration of 12.5 mg test item/L. At the concentration of 25 mg test item/L, 4 animals were immobile and 20 animals were immobile at the concentration of 50 and 100 mg test item/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 value was calculated to be 30.5 mg test item/L and the 48-h LOEC was determined to be 25 mg test item/L. Based on the experimental results, the 48-h NOEC was determined to be 12.5 mg test item/L.