REAKTIVE GELB F 97494

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From February 19, 2002 to February 21, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Sampling schedule for chemical analysis: at 0 and 48 h

Vehicle:

no

Details on test solutions:

Test solution: To produce the only test concentration, 105.3 mg of the test substance was added to 1 L of dilution water and treated with a magnetic stirrer.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Sex: parthenogenetic females
- Source: strain of Bundesgesundheitsamt Berlin
- Age at study initiation: neonates
- Food type: green algae (Desmodesmus subspicatus)
- Frequency:ad libitum

ACCLIMATION
A population of parthenogenetic females of synchronized age structure which had been maintained for more than 15 years in the test facility under constant temperature conditions (20±1°C) at a 16:8 h light-dark photoperiod (illumination: < 1000 lux) were used in the study. The culture water (so-called 'M4 medium') was partly renewed once a week. The Daphnia were exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

271.3 mg CaCO3/L

Test temperature:

18-22°C

pH:

7.7

Dissolved oxygen:

Control: 8.5 mg/L
Test: 8.6 mg/L

Nominal and measured concentrations:

Nominal concentration: 100 mg/L

Measured concentration: measurement was carried out in terms of TOC
0 h = 106% of nominal (= 70 mg/L of organic components of the test substance)
48 h = 106% of nominal (= 70 mg/L of organic components of the test substance)
The analytical values indicate a good recovery rate.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL glass beakers holding 10 neonates in 20 mL of test medium
- Type: closed bottle
- Aeration: none
- Type of system: static
- No. of organisms per vessel: 10 neonates per vessel
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 2 mL per neonate

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water ('M4 medium'), originally described in Water Research 24 (9): 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin
- Total organic carbon: <2 mg/L
- Timepoint of water quality measurement: 48 h

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark

- Test concentrations: 100 mg/L (nominal)

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 106 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 106 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Immobilization in control: 0

Time [h]	EC 50 [mg/L] (test substance)
24	≥100
48	≥106

The results are expressed in terms of nominal concentrations (at 24 h), and in terms of mean measured concentrations (at 48 h). Measured concentrations ranged from 106 (at 0 h) to 106% (at 48 h) of nominal value.

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 48 h EC50 and NOEC of the test substance were found to be ≥100 mg/L.

Executive summary:

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2 (equivalent or similar to OECD Guideline 202), in compliance with GLP.

In this test, 2 replicates of 10 Daphnia magna neonates were exposed to a nominal concentration of 100 mg/L under static conditions for 48 h. Immobility was recorded at 24 and 48 h. Measured concentrations were equivalent to ca. 106% of nominal values at both 0 and 48 h.

Under the test conditions, the 48 h EC50 and NOEC of the test substance were ≥100 mg/L (nominal concentration).

Description of key information

Under the test conditions, the 48 h EC50 and NOEC of the test substance were found to be ≥100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L

Additional information

A limit test was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2 (equivalent or similar to OECD Guideline 202), in compliance with GLP. In this test, 2 replicatesof 10 Daphnia magna neonates were exposed to a nominal concentration of 100 mg/L under static conditions for 48 h.Immobility was recorded at 24 and 48 h. Measured concentrations were equivalent to ca. 106% of nominal values at both 0 and 48 h. Under the test conditions, the 48 h EC50 and NOEC of the test substance were ≥100 mg/L (nominal concentration).