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EC number: 250-954-9 | CAS number: 32210-23-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- From 05.03.1985 to 07.03.1985
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- This study was performed according to DIN 38412 part 15 without GLP statement. Solvent was used. The test duration was only 48 hours. Analytical measurements were not performed. Some information are missing to validate this study, such as information on the test substance and mortality in control.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38412 Part 15
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- no analytical monitoring
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Test organisms (species):
- Leuciscus idus melanotus
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- none
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 13-16 mg/L
- Sublethal observations / clinical signs:
Table 6.1.1/1: Raw data
Nominal concentration (mg/L)
48 hours
Surviving
Dead
% mortality
10
13
16
20
10
9
2
0
0
1
8
10
0
10
80
100
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48h-LC50 was determined to be 15 mg/L (95% Confidence limit at 13-16 mg/L).
- Executive summary:
This study was performed according to DIN 38412 Part 15 without GLP statement, to assess the acute toxicity of the test substance to fish Leuciscus idus melanotus, under static conditions.
Fish were exposed to 4 nominal test substance concentrations (10, 13, 16 and 20 mg/L) with solvent during 48 hours and the mortality was recorded.
The 48h-LC50 was determined to be 15 mg/L (95% Confidence limit at 13 -16 mg/L).
Some information are missing to validate this study, such as mortality in control. Validity criteria cannot be verified.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 27.01.1997 to 21.02.1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was performed according to EU Method C.1 with GLP statement. All validity criteria were fulfilled and no deviations from the protocol were observed. This study is well documented.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- A series of concentrations in deionised water (2 stock solution representing the 2 test: at 33.7 mg/L and 29.8 mg/L, test concentrations at 2.8, 5.7, 8.5, 14.2, 24.1 and 28.4 mg/L) was prepared. The analytical monitoring was carried out at 0, 24, 48 and 72 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method for stock solution: 1 g/L in drinking water, stirred for ca. 18 hours and then filtered.
- Eluate: Drinking water
- Controls: one control medium
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Cyprinus carpio
- Source: Bio International B.V; Someren NL
- Length at study initiation (length definition, mean, range and SD): 3-4 cm
- Feeding during test: no
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): same as test (in dechlorinated drinking water in 200-300L glass aquaria at 20 +/- 2°C).
- Type and amount of food: TRUOVIT, amount c.a 1% bodyweight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): No disease, <5% mortality.
QUARANTINE (wild caught)
- Duration: 14 days - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- None
- Hardness:
- ca. 13 °dH.
- Test temperature:
- 19.6-20.7°C.
- pH:
- 8.0-8.4
- Dissolved oxygen:
- 90-100% saturation
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- - Nominal concentrations: 6.0, 7.5, 9.5 and 12 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: ca. 20 L
- Aeration: continuously
- Renewal rate of test solution (frequency/flow rate): daily change
- No. of organisms per vessel: 10 fish
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): not applicable
TEST MEDIUM / WATER PARAMETERS
Drinking water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8h
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.2
- Range finding study: yes
- Test concentrations of the range finding study: 4.0, 6.7, 12, 20 and 34 mg/L.
- Results used to determine the conditions for the definitive study: See table 6.1.1/1 in "Any other information on materials and methods incl. tables". - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 7.7-9.6 mg/L
- Details on results:
- - Analytical monitoring: See table 6.1.1/2 in "Any other information on results incl. tables". The measured values after 72 hours were not greater by more than 20% of the fresh concentrations. Based on this information, the results were based on nominal concentration only.
- Raw data: See table 6.1.1/3 in "Any other information on results incl. tables".
- Mortality of control: 10% of mortality at the end of the test (96h). - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- None
- Sublethal observations / clinical signs:
Table 6.1.1/2: Analytical monitoring
Stability control (mg/L)
Actual concentration at 0h (mg/L)
Measured concentration at 24h (mg/L)
Measured concentration at 48h (mg/L)
Measured concentration at 72h (mg/L)
2.8
5.7
8.5
14.2
24.1
28.4
2.4
5.3
8.1
14.4
23.5
28.4
3.0
4.9
7.4
12.5
21.9
25.1
3.0
5.4
8.3
14.1
22.0
28.6
3.1
5.3
7.7
13.8
21.9
31.9
Stock solution - Range finding test
33.7
31.7
28.3
25.6
Stock solution - test
29.8
24.6
29.0
29.8
Table 6.1.1./3: Raw data
Nominal concentration (mg/L)
24 hours
48 hours
72 hours
96 hours
Surviving
Dead
% mortality
Surviving
Dead
% mortality
Surviving
Dead
% mortality
Surviving
Dead
% mortality
Control
6.0
7.5
9.5
12
10
10
10
6
0
0
0
0
4
10
0
0
0
40
100
10
10
10
3
0
0
0
0
7
10
0
0
0
70
100
10
10
9
3
0
0
0
1
7
10
0
0
10
70
100
9
10
8
3
0
1
0
2
7
10
10
0
20
70
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96h-LC50 was determined to be 8.6 mg/L (95% Confidence limit at 7.7 -9.6 mg/L).
- Executive summary:
This study was performed according to EU Method C.1 with GLP statement, to assess the acute toxicity of the test substance to fish Cyprinus carpio, under semi-static conditions.
A stability test was performed to measure the concentrations of the test substance in aqueous solution during 72 hours. Results show that the test substance is stable during the test duration.
After a range-finding test, fish were exposed to 4 nominal test substance concentrations (6.0, 7.5, 9.5 and 12 mg/L) with a control medium during 96 hours and the mortality were recorded every day.
The 96h-LC50 was determined to be 8.6 mg/L (95% Confidence limit at 7.7 -9.6 mg/L). The 96h-LC0 and 96h-LC100 were 6.7 and 12 mg/L, respectively.
All validity criteria were fulfilled while mortality in the control was 10% after 96 hours.
Referenceopen allclose all
Description of key information
EU Method C.1, GLP, key study, validity 1:
96h-LC50 = 8.6 mg/L (95% confidence limit = 7.7-9.6 mg/L).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 8.6 mg/L
Additional information
To assess the short-term toxicity of the registered substance to fish, two studies are available. Among this data, one study is assessed as the key study (Scholz, 1997) and the other study as supporting (Scholz, 1996).
According to the key study, performed according to EU Method C.1 with GLP statement, the fish Cyprinus carpio were exposed to a range of substance concentrations (6.0 to 12.0 mg/L) under semi-static conditions, during 96 hours. The 96h-LC50 based on mortality was determined to be 8.6 mg/L (95% confidence limit at 7.7 -9.6 mg/L).
In the supporting study, solvent was used, the test duration was only 48 hours and no analytical measurements were performed. Furthermore, some information were missing and validity criteria cannot be verified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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