Registration Dossier
Registration Dossier
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EC number: 212-485-8 | CAS number: 822-06-0
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
In an Ames test with the S. typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 1,6-hexamethylene diisocyanate (HDI; vapour-phase exposure) revealed no mutagenic activity in the absence and in the presence of a metabolic activation system (Wagner and Klug, 1998; Wagner et al., 2000). Using vapor-phase exposure HDI did not induce mutagenic effects in the in vitro gene mutation assay (HGPRT test) with Chinese hamster ovary (CHO) cells in the presence and absence of a metabolic activation system (San and Clarke, 1998; Wagner et al., 2000).
In a micronucleus test (MNT) equivalent to OECD TG 474 male and female mice were once whole-body exposed for 6 hours to HDI vapour concentrations of 0.14, 0.80 and 1.47 ppm. No significant increase in micronucleated polychromatic erythrocytes in test substance treated groups relative to the respective air control group was observed in male or female mice at 24 or 48 hours after exposure. Therefore, HDI was concluded to be negative in the mouse micronucleus assay after inhalation exposure (Gudi and Krsmanovic, 1998; Wagner et al., 2000).
Justification for classification or non-classification
Genetic toxicity (in vitro and in vivo)
Not classified under Annex I of Directive 67/548/EEC. According to Annex I of Regulation (EC) No 1272/2008 no classification is required for genetic toxicity.
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