slag, copper smelting

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN EN ISO 11348-2

Principles of method if other than guideline:

A suspension of bioluminiscent bacteria is brought into a medium containing a specific concentration of copper slag extracts. After 30 minutes, the inhibition of bioluminiscence is assessed.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Iron silicate, copper smelting and refining

EC Number:

701-480-0

Cas Number:

67711-92-6

IUPAC Name:

Iron silicate, copper smelting and refining

Details on test material:

Slow cooled copper slag (stones) with the following chemical composition:
Fe 40.01%
SiO2 32.12 %
Cu 9300 mg/kg
Pb 4150 mg/kg
Zn 25100 mg/kg
As 12 mg/kg
Ni < 5 mg/kg
Co 310 mg/kg
Cd 17 mg/kg
Sn 1545 mg/kg

Sampling and analysis

Details on sampling:

at Tend

Test solutions

Details on test solutions:

Eluates prepared in ratio 1 /10 ( 100 g sample in 1000 ml water ) according to DIN 38414
Concentrations in eluate ( mg/l)
As 0.027
Ba 0.11
Ca 14
Cd <0.0001
Co <0.01
Cr<0.1
Cu 0.02
Hg < 0.0002
Mo 0.12
Ni < 0.01
Pb < 0.027
Sb 0.004
Tl <0.0005
Zn 0.01

Test organisms

Test organisms (species):

Vibrio fisheri

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test conditions

Hardness:

DOC 1.43 mg/l

pH:

9.9

Results and discussion

Effect concentrations

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 80 g/L

Conc. based on:

test mat.

Basis for effect:

other: bioluminescence

Any other information on results incl. tables

Concentration of the eluate in test concentration	Eluate (1-10)
Luminescent bacteria toxicity, GI - value	1

 

*Smallest dilution factor that results in a 20% light emission reduction

EC 20

> 800 ml eluate/L

 

The above values can be converted to toxicity for copper slag by taking into account the amount of slag used to produce the eluate : 100 g slag / 1000 ml water

EC 20

> 80 g copper slag /L

Applicant's summary and conclusion

Conclusions:

An EC20 value of >80 g/L was observed.

Executive summary:

Effect of water extracts of copper slags on the bioluminescence of Vibrio fischeri was assessed during a 30 min experiment. The test was performed according to DIN EN ISO 11348 -2. An EC20 value of >80 g/L was observed.