Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-129-9 | CAS number: 2004-62-8
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The testing was conducted between 29 June 2009 to 28 July 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Fully GLP and test guideline compliant study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 429 (Skin Sensitisation: Local Lymph Node Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 3,3'-(hexane-1,6-diyldiimino)dipropanenitrile
- EC Number:
- 700-129-9
- Cas Number:
- 2004-62-8
- Molecular formula:
- C12H22N4
- IUPAC Name:
- 3,3'-(hexane-1,6-diyldiimino)dipropanenitrile
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Laboratories UK Ltd, Bicester, Oxon, UK
- Age at study initiation: 8 to 12 weeks old
- Weight at study initiation: 15 to 23 g
- Housing: Individually housed in suspended solid-floor polypropylene cages furnished with softwood woodflakes.
- Diet (e.g. ad libitum): Free access to 2014 Teklad Global Rodent diet supplied by Harlan Teklad, Blackthorn, Bicester, Oxon, UK
- Water (e.g. ad libitum): Free access to mains tap water.
- Acclimation period: At least 5 days.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 to 25 oC
- Humidity (%): 30 to 70%
- Air changes (per hr): approx. 15 changes per hour.
- Photoperiod (hrs dark / hrs light): 12 hours continuous light (06.00 to 18.00) and 12 hours darkness.
IN-LIFE DATES: From: 29.06.2009 To: 28.07.2009
Study design: in vivo (LLNA)
- Vehicle:
- dimethylformamide
- Concentration:
- 25%, 10% and 5% in dimethyl formamide.
- No. of animals per dose:
- Four animals per dose.
Results and discussion
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Value:
- 27.44
- Test group / Remarks:
- 4
- Remarks on result:
- other: see Remark
- Remarks:
- Concentration (% v/v) in dimethyl formamide: 5 Result: Positive
- Parameter:
- SI
- Value:
- 41.49
- Test group / Remarks:
- 4
- Remarks on result:
- other:
- Remarks:
- Concentration (% v/v) in dimethyl formamide: 10 Result: Positive
- Parameter:
- SI
- Value:
- 71.34
- Test group / Remarks:
- 4
- Remarks on result:
- other:
- Remarks:
- Concentration (% v/v) in dimethyl formamide: 25 Result: Positive
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (skin sensitising) based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- The test material was considered to be a sensitiser under the conditions of the test.
- Executive summary:
The test material was considered to be a sensitiser under the conditions of the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
