Octane-1,2-diol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to international guideline(s), GLP-compliant, performed in recognized contract research organization, no restrictions, fully adequate for assessment.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

;2004

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Version / remarks:

;1998

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock solution of 1000 mg/L was prepared in test medium. The stock solution was stirred at 30°C for 2 hours.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: in-house culture originating from Daphnia magna Straus collected in Langedam, Birkerød, Denmark; cultured at DHI since 1979
- Age at study initiation: < 24 h
- Feeding during test: not indicated

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

250 ± 25 mg/L as CaCO3

Test temperature:

20 °C ± 0.4 °C

pH:

7.7 - 7.9

Dissolved oxygen:

95 - 99 % of saturation

Nominal and measured concentrations:

nominal concentrations: 10, 20, 50, 100, 200, 500, and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass containers containing 25 mL of test solution
- Renewal rate of test solution (frequency/flow rate): after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- Biomass loading rate: 5 mL medium per organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deinonised water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: the test was performed in total darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : numbers of immobile animals was recorded 24 hours (before and after changing the test medium) and 48 hours after the start of the study

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0-2.5
- Range finding study: not reported

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

176 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: upper 95% confidence limit: 193 mg/L; lower limit not calculabe

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

146 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: upper 95% confidence limit: 178 mg/L;(lower limit not calculabe

Details on results:

- Immobilised Daphnia: see Table 1
- Mortality of control: none

Results with reference substance (positive control):

- Results with reference substance valid? Yes (range specified in ISO 6341 (0.6-2.1 mg/L)
- EC50/LC50: 24-hour EC50: 1.9 mg/L (95% confidence limits 1.7-2.2 mg/L).

Reported statistics and error estimates:

The EC10 and EC50 values were calculated by Probit Analysis.

Table 1: Cumulative immobilisation

 

Nominal concentration (mg/L)	Cumulative numbers of immobile Daphnia
	24 hours		48 hours
	total	%	total	%
Control	0	0	0	0
10	0	0	0	0
20	0	0	0	0
50	0	0	0	0
100	0	0	0	0
200	9	45	17	85
500	20*	100	20	100
1000	20**	100	20	100

*: 90% of the test organisms were immobile after 20 min

**: 100% of the test organisms were immobile after 20 min

Validity criteria fulfilled:

yes

Remarks:

In the control, no Daphnia have been immobilised. The dissolved oxygen concentration at the end of the test was ≥3 mg/L (≥60% of the air saturation value at the temperature used).

Description of key information

48-h EC50 > 100 mg/L for Daphnia magna (OECD TG 202, ISO 6341))