Hexan-1-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1965

Report date:

1965

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

other: LD50

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hexan-1-ol

EC Number:

203-852-3

EC Name:

Hexan-1-ol

Cas Number:

111-27-3

Molecular formula:

C6H14O

IUPAC Name:

hexan-1-ol

Test animals

Species:

rat

Strain:

other: Holtzman albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Weight at study initiation: 200-265g

- Fasting period before study: Over night

- Housing: Group housing (5 of each sex per cage), in metal cages provided with white pine and cheddar shavings.

- Diet: Purina Laboratory Chow, ad libitum.

- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: Exploratory doses were administered to 8 rats to estimate the order of toxicity of the test compound. Based on the preliminary estimation, groups of 10 rats (5M, 5F) were administered the test compound at graded dosage levels designed to blanket the toxicity range.

Doses:

1.17, 1.65, 2.33, 3.28, 4.64 and 6.55 gm/kg

No. of animals per sex per dose:

5 female, 5 male

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

- Frequency of observations and weighing: Regular intervals on the day of dosing and daily thereafter for 14 days.

- Necropsy of survivors performed: yes

- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: Gross necropsies were performed on all  survivors and any animals which died during the observation period. Body weights of survivors were recorded prior to sacrifice.

Statistics:

Confidence Interval 2.85 ml (2.35 gm) to 5.34ml (4.39gm)/kg body weight. Slope function 1.88, with confidence interval of 1.26 to 2.80.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 210 mg/kg bw

Mortality:

All deaths occurred within 24 hours of dosing. See table. 

.

Clinical signs:

other: Animals at all dose levels exhibited weakness and ataxia. They became comatose and breathing was laboured while comatose.  Animals  which survived appeared normal within 24 hours other than top dose animals (6.55 g/kg) where the rats appeared unwell up t

Gross pathology:

Necropsy of animals which died showed congestion of  the lungs and adrenals in most animals. In some cases gastric 
congestion  was also observed. There were no remarkable gross findings in animals sacrificed at the end of the observation period.

Other findings:

- Organ weights: Not recorded

- Histopathology: Not available

- Potential target organs: No conclusion drawn

- Other observations: No sex specific differences were reported, mortality represented as a combined value so no independent assesment can be made.

Any other information on results incl. tables

Table 1: Number of animals dead within the 14 day study period.

 

Dose ml (and gm.) kg./body weight	Mortality (# dead/total)			Time of death (day)
	Male	Female	Combined
1.42 (1.17)			0/10
2.00 (1.65)			1/10	1
2.83 (2.33)			4/10	1
3.99 (3.28)			410	1
5.64 (4.64)			8/10	1
7.96 (6.55)			8/10	1

 

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The acute oral toxicity study for hexan-1-ol, conducted according to a guideline similar to the now-deleted OECD Test Guideline 401 but prior to GLP compliance, reports an LD50 value of 3210 mg/kg in rat.