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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not inherently biodegradable: 8% in 28 days  (% of ThOD),  COD: 2.50 mg O2/mg test. mat. (OECD 302 C)

Key value for chemical safety assessment

Additional information

Phenol 2,6 -bis(1 -methylpropyl) was evaluated for its inherent biodegradability. The test substance attained 8% degradation after 28 days calculated from oxygen uptake (% of ThOD) and so, therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines. It cannot be excluded that at the test concentration of 30 mg/l, there was already some bacterial toxicity that may have influenced the study outcome.

Test method:

The method used to test the inherent biodegradability followed that described in the OECD Guidelines for Testing of Chemicals / Section 2: Degradation and Accumulation, Test No. 302C "Inherent Biodegradability, Modified MITI Test (II). A mixed population of activated sewage sludge microorganisms was used as inoculum and aniline as reference substance. Initial concentration of test substance was 30 mg/l and reference substance 100 mg/l. TOC analysis of phenol 2,6 -bis(1 -methylpropyl) was not possible due to the insoluble nature of the test material. A modified Miti test was carried out due to this fact. A COD value of 2.50 mg O2/mg was obtained for the test material.