4-isopropylaniline

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP Guideline Study, limited documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Principles of method if other than guideline:

Guideline 67/548/EWG (C.3)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-isopropylaniline

EC Number:

202-797-2

EC Name:

4-isopropylaniline

Cas Number:

99-88-7

Molecular formula:

C9H13N

IUPAC Name:

4-(propan-2-yl)aniline

Details on test material:

- Name of test material (as cited in study report): p-isopropylanilin
- Analytical purity: 99.8
- Lot/batch No.: 04012348 (Tank 505)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: stock solution (62.5 mg/L)

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: Scenedesmus subspicatus CHODAT
- Source: strain obtained from the Institute of Plant Physiology at the University of Göttingen (Germany)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

ca. 23 °C

pH:

8.2 - 8.4 (0 h)
7.4 - 10.4 (after 72 h)

Nominal and measured concentrations:

Nominal concentrations: Control, 1.6, 3.1, 6.3, 12.5, 25, 50 mg/L
Measured concentrations (Initial): < 2, 2, 4, 6, 11, 20, 39 mg/L
Measured concentrations (72h): < 2, 8, 6, 9, 12, 19, 39 mg/L
Measured concentrations refers to TOC, 1 mg/L TOC equates to 1.3 mg/L test substance

Details on test conditions:

TEST SYSTEM:
- static test system
- test vessel: 300 ml Erlenmeyer flasks with ground glass stoppers
- test cultures: final cell density 10 E+4 cells/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline (Millipore-water)

OTHER TEST CONDITIONS
- Photoperiod: continuous uniform illumination (spectral range 400 to 700 nm)
- light intensity: 120 µE / m² x s

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: Cell counting with Microcellcounter Sysmex F-300 Digitana

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

5.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

4.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

9.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 4 -isopropylaniline to algae was performed according to the Guideline 67/548/EWG (C.3) in a static test system. For a test period of 72 hours an EC50 value of 13 mg/l based on nominal concentrations was reported for growth rate (Bayer AG, 1997).