Guaiacol

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study was not a standardised method but it was well described

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Acute toxicity of substituted phenols to Rana japonica tadpoles and mechanism-based quantitative structure-activity relationship (QSAR) study

Author:

Wang X., Dong Y., Wang L. and Han S.

Year:

2001

Bibliographic source:

Chemosphere, 44 : 447-455

Reference 2

Reference Type:

publication

Title:

Quantitative structure-activity relationships for the toxicity to the tadpole Rana japonica of selected phenols

Author:

Wang X., Dong Y., Xu S., Wang L., and Han S.

Year:

2000

Bibliographic source:

Bull. Environ. Contam. Toxicol., 64 : 859-865

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

evaluate the toxicity on Tadpoles at 12h and 24h.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Guaiacol

EC Number:

201-964-7

EC Name:

Guaiacol

Cas Number:

90-05-1

Molecular formula:

C7H8O2

IUPAC Name:

Phenol

Details on test material:

It was provided by Department of Chemistry at Nanjing University and was of sufficient purity (analytical purity). Further purification was not necessary.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: All exposures took place at water temperature 22-25°C in a controlled environmental chamber (pH = 6.31). Aerated chloride-free water was used for preparing the stock solutions of chemical and test dilution.
A six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.

Test organisms

Test organisms (species):

Rana sp.

Details on test organisms:

TEST ORGANISM
- Common name: tadpoles
- Strain: Rana Japonica
- Source: collected from a permanent pond in Nanjing.
- Age at study initiation: 30 day-old
- Weight at study initiation: 0.09 +/- 0.01 g
- Length at study initiation: 2.5 +/- 0.1 cm
- Method of breeding: eggs and the resulting tapdoles were cultured in aerated stream water (Cl-: 3.40 mg/L, pH: 7.74, dissolved oxygen concentration: 9.6 mg/L, Na+: 2.35 mg/L; Ca2+: 48 mg/L) in the laboratory at romm temperature (22-25°C).
- Feeding during test
- Food type: an excess of rinsed, salt free canned spinach
- Amount: no data
- Frequency: daily

ACCLIMATION: none

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

12 h

Remarks on exposure duration:

12 and 24 hours

Test conditions

Test temperature:

22-25°C

pH:

measured

Nominal and measured concentrations:

a six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: 1000 ml pyrex beaker containing 500 ml test solution or aerated chloride-free water.
- Aeration: no
- Renewal rate of test solution: every 6 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS: aerated chloride-free water

OTHER TEST CONDITIONS:
- Photoperiod: 14 (day) /10 (night) hours.

EFFECT PARAMETERS MEASURED: mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration were set after range finding experiments.
- Range finding study: no data

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

12 h

Dose descriptor:

LC50

Effect conc.:

430.44 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

392.57 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Reported statistics and error estimates:

Logarithms of inverse of median lethal concentrations after 12-h and 24-h exposure were calculated using non-linear regression in STATISTICA software.