Benzene, 1-(cyclopropylmethyl)-4-methoxy-

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

444-110-0

EC Name:

-

Cas Number:

16510-27-3

Molecular formula:

C11H14O

IUPAC Name:

1-(cyclopropylmethyl)-4-methoxybenzene

Details on test material:

Batch No LDP0200015
Purity 99.1%

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

Experiment I : 10;33;100;333;1000; and 2500 microg/plate
Experiment II : 3;10;33;100;333 and 1000 microg/plate

Controls

Controls 1

Untreated negative controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

Negative solvent / vehicle controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

True negative controls:

no

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

TA1535 and TA100 with a concentration of 10 microg/plate without metabolic activation

Controls 2

Untreated negative controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

Negative solvent / vehicle controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylene-diamine

Remarks:

TA1537 and TA98 with a concentration of 10 microg/plate and 50 microg/plate respectively without metabolic activation

Controls 3

Untreated negative controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

Negative solvent / vehicle controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

True negative controls:

no

Positive controls:

yes

Positive control substance:

methylmethanesulfonate

Remarks:

TA102 with a concentration of 4 microg/plate without metabolic activation

Controls 4

Untreated negative controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

Negative solvent / vehicle controls:

yes

Remarks:

Concurrent untreated and solvent controls were performed

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

TA1535, TA1537, TA98, TA100 and TA102 with a concentration of 2.5 microg/plate with metabolic activation

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

toxic effects, evident as a reduction in the number of revertants was observed at the higher concentration with and without metabolic activation in all strains used.

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Therefore Cyclopropenyl Estragole is considered to be non-mutagenic in this Samonella Typhimurium reverse mutation assay.