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EC number: 434-430-9 | CAS number: -
Toxicity of EA-3098
The table below presents:
- oxygen concentration at the start of the measurement
- the influence of controls and EA-3098 on the oxygen consumption of microbes in activated sludge
- the percentage inhibition of the respiration rate
Oxygen conc. At the start (mg O2/l)
Oxygen consumption mg O2/l/hr
Mean C1 + C2
* Negative values indicate stimulation of respiration rate of the sludge. These values are considered as not significant.
No inhibition of respiration rate of the sludge was recorded at 100 mg EA-3098 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore, no further testing was needed. Hence, the EC50 of EA-3098 exceeded 100 mg/l (based on nominal concentrations).
Toxicity of the reference substance (3,5 -dichlorophenol)
Mean C2 + C3
The calculated percentages inhibition allowed for a reliable determination of an EC50 -value (see Appendix 1 - attached background material).
Acceptability of the test:
1. The mean respiration rate of control 1 and 2 was 46 mg O2/l/hr. The difference between the controls was within 15% of each other (2%).
2. The mean respiration rate of control 2 and 3 was 49 mg O2/l/hr. The difference between the controls was within 15% of each other (10%).
3. The EC50 of 3 ,5 -dichlorophenol was in the accepted range of 5 -30 mg/l.
Since all criteria were met, the study was considered valid.
The influence of the test material on the respiration rate of activated sludge was investigated after a contact time of 30 minutes.
The study procedure was based on OECD Guideline No. 209, EEC Directive 67/548 and ISO Standard 8192.
The test material was a white powder and was treated as 100% pure. Since the test material is poorly soluble in water, it was quantitatively added to the test vessels. A nominal concentration of 100 mg/l was tested in duplicate. Optimal contact between the test substance and the test medium was ensured by applying continuous aeration and stirring.
No inhibition of respiration rate of the sludge was recorded at 100 mg/l of test material. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, the study was considered to be valid.
In conclusion, under the conditions of the test, EA-3098 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
Under the conditions of the test, EA-3098 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
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