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EC number: 443-510-2 | CAS number: 738587-10-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study test phase 2002-10-28 to 2002-11-01. Study report 2002-12-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 443-510-2
- EC Name:
- -
- Cas Number:
- 738587-10-5
- Molecular formula:
- C26H14N10Ni2O18S4 as free acid
- IUPAC Name:
- dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The study was run with nominal concentrations of 1500 mg/L of test material together with a dilution water control.
Test organisms
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Mirror carp
- Source: Reputable supplier
- Length at end of study: 36-40mm, mean 38mm
- Weight at end of study: 1.21-2.57g, mean 1.87g
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7d
- Acclimation conditions: 22 +/- 2 degrees C
- Type of food: commercial fish food (food was witheld at least 24h prior to commencement of test)
- Health during acclimation:No mortalities observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Hardness of dilution water 41.7 mg/L CaCO3
- Test temperature:
- 21.8-22.2 degrees C
- pH:
- 7.7-8.3
- Dissolved oxygen:
- 89-94%
- Salinity:
- Salinity of dilution water 1.0
- Nominal and measured concentrations:
- The overall mean measured concentration was 107% of the nominal value. The percentage loss in the measured concentrations over the test period was <1%. On the basis of the analytical data the nominal concentrations were used for the calculation and reporting of results.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Borosilicate glass
- Fill volume: Vessels had a max. capacity of 40L and a working volume of 25L
- Aeration: Test solutions were gently aerated
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- Biomass loading rate: 0.75g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water which has been passed through activated carbon, coarsely filtered, dechlorinated, salts added (to maintain hardness), UV sterilised and re-filtered (5um).
- Chlorine (as Cl2): Free residual <2 ug/L Combined residual <2 ug/L
- Alkalinity: 20.4 mg/L (as CaCO3)
- Conductivity: 243 uS/cm (at 25 degrees C)
- Intervals of water quality measurement: Daily measurements of the test solutions for pH, temperaure and dissolved oxygen. The temperature of the dilution water control was measured hourly.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours fluorescent light and 8 hours dark with a 20 min. dawn/dusk transition period.
EFFECT PARAMETERS MEASURED: Observation for mortalities were made at 3, 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Test concentrations: 1500 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 500 mg/L
- Details on results:
- Due to the intensity of colour in the test solution it was not possible to assess symptoms of toxicity during the study.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No mortalities were observed in either the control or the 1500 mg/L test concentration vessel during this study. Therefore, the 96h LC50 was determined to be >1500 mg/L. The No Observed Effect Concentration (NOEC) was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.
- Executive summary:
Introduction
The study was undertaken to determine the acute toxicity of the test substance to mirror carp Cyprinus carpio. The method was designed to be compatible with the following guidelines:
OECD Guidelines for the Testing of Chemicals (1992). Method 203. Fish, Acute Toxicity Test.
Official Journal of the European Communities, L 383 A, Part C1, Acute Toxicity for Fish (29th December 1992).
Results & Conclusions
During this study there were no mortalities observed in both the dilution water control vessel and the test substance vessel. Therefore the 96h LC50 was determined to be >1500 mg/L. The 96h NOEC was determined to be 1500 mg/L. The lowest nominal concentration at which there was 100% mortality was >1500 mg/L.
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