Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2 June 1993 - 18th June 1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

EC Number:

412-780-3

EC Name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Cas Number:

1380226-46-9

Molecular formula:

A complex mixture of species so no unique molecular formula can be given

IUPAC Name:

dimolybdenum(6+) diammonium 2-{2-[(octadec-9-en-1-yl)amino]ethoxy}ethan-1-ol heptaoxidandiide

Test material form:

liquid: viscous

Details on test material:

- Name of test material: Additiv 104-Konzentrat
- Description: Dark red liquid
- Purity: Product by process
- Lot/batch No.: DS 147
- Expiration date: March 9, 1994
- Storage condition of test material: Room temperature in the dark
- Stability: Stable

Method

Species / strain

Species / strain / cell type:

bacteria, other: Salmonella Typhimurium TA 1535, TA 1537, TA 98, TA 100 Escherichia coli WP2, WP2 uvrA

Metabolic activation system:

Aroclor induced S9 rat liver fraction.

Test concentrations with justification for top dose:

Concentration range in the main test (with metabolic activation): 33 ... 5000 µg/plate
Concentration range in the main test (without metabolic activation): 33 ... 5000 µg/plate

Vehicle / solvent:

Solvent: DMSO/dichloromethane 9/1

Results and discussion

Test results

Test results 1

Species / strain:

other: as specified above

Metabolic activation:

with

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

( 2500 µg/plate)

Test results 2

Species / strain:

other: as specified above

Metabolic activation:

without

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

( 2500 µg/plate)

Remarks on result:

other: other: preliminary test

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

Executive summary:

In a GLP compliant, guideline bacterial reverse mutation assay the test substance did not induce point mutations by base pair changes or frameshifts in the genome of the strains used. Therefore, the test susbtance is considered to be non-mutagenic in this Salmonella typhimurium and Escherichia coli reverse mutation assay.