Registration Dossier
Registration Dossier
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4-amino-5-hydroxy-6-(5-{4-chloro-6-[4-(2-sulfonatooxyethanesulfonyl)phenylamino]-1,3,5-triazin-2-ylamino}-2-sulfonatophenylazo)-3-(2-sulfonato-4-(2-sulfonatooxyethanesulfonyl)phenylazo)naphthalene-2,7-disulfonate potassium/sodium;reaction mass of: 4-amino-3-(4-ethenesulfonyl-2-sulfonatophenylazo)-5-hydroxy-6-(5-{4-chloro-6-[4-(2-sulfonatooxyethanesulfonyl)phenylamino]-1,3,5-triazin-2-ylamino}-2-sulfonatophenylazo)naphthalene-2,7-disulfonate potassium/sodium
EC number: 451-440-9 | CAS number: 586372-44-3
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Effect on Survival and Reproduction of Daphnia magna in a Semi-Static Test over Three Weeks. Experimental test performed under GLP conditions and according to OECD Guidelines 211 and method from the EC Directive 92/69/EEC, C.20.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 3.2 mg/L
Additional information
Taking into account the effects on survival and reproduction of the test animals, the highest concentration of the substance tested without toxic effects after the exposure period of 21 days (21-day NOEC) was 3.2 mg/L.
The lowest concentration tested with toxic effects (21-day LOEC) was determined to be 10 mg/L due to the statistically significantly reduced mean reproduction rate of Daphnia magna at this test concentration.
The 21 -day EC50 for the inhibition of the reproduction rate of Daphnia magna was calculated to be 34.8 mg/L (95% confidence limits: 30.1-41.9 mg/L).
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