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EC number: 427-430-5 | CAS number: 54301-26-7
Inhibition of respiration in activated sludge, in compliance with OECD guideline n°209 and EEc directive 87/302
-micro-organisms from activated sludge (800 mg of suspended solids per litre of medium) were exposed to a range of concentrations of test substance for 3 hours; 1, 10 , 100mg test substance per litre of test medium
-two contro media (without test substance) were tested, one at the beginning of the test, the other one at the end of the test
-the dissolved oxygen concentration was measured in 300 mL samples from each after approximately 3 hours exposure
-a reference substance, 3,5 -dichlorophenol, was tested under the same conditions in order to control the sensitivity of the activated sludge (3 concentrations)
-the criteria of quality for the study, as notified in the OECD and EEC standards and relative to the level of oxygen consumption in the two control media and to the toxicity of 3,5 -dichlorophenol, were respected
Taking into account the results obtained under the experimental conditions employed, these ones may be expressed as follows:
EC50 -3 hours higher than 100 mg/L
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