Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 480-390-0 | CAS number: -
The analytically determined concentrations of Mn and Ni in the test material solutions were:
Mn content: 18.4 g/100 g
Ni content: 19.7 g/100 g
Table 1: Analytically measured concentrations - unfiltered
Nominal loading rate (mg/L)
Table 2: Analytically measured concentrations - filtered (0.2 µm)
Filtered and unfiltered samples were analysed with nearly identical results, demonstrating the absence of undissolved test material in the test solution. Mn concentrations were below the LOQ at the start and end of exposure. Ni concentrations were stable over the exposure period at 0.06 mg/L. However, the estimated equivalent amount of test material in solution was < 0.5 mg/L. Nevertheless, the test solutions were considered saturated with all soluble components of the test material, in test media and under test conditions.
All reasonable efforts were used to generate a saturated solution of the test material in test media following the guidance in OECD 23 and OECD 29. Since the test material is a mixed metal oxide, the test solution is considered a water accommodated fraction (WAF).
Table 3: Mortality and toxic signs
Number of fish
Cumulative mortality (number of dead fish)
Observed signs of toxicity
The short term toxicity of the test material to freshwater fish was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 203, EU Method C.1, US EPA OPPTS 850.1075 and EPA OPP 72 -1.
During the study, groups of 10 Pimephales promelas were each exposed to test material at nominal concentrations of 0 (dilution water control) and 100 mg/L, under static conditions, for 96 hours. Mortality, adverse effects and any signs of abnormal behaviour were recorded.
The content of manganese and nickel in the test solutions was analysed during the study. Filtered and unfiltered samples were analysed with nearly identical results. Manganese concentrations were below the LOQ at the start and end of exposure. Nickel concentrations were stable over the exposure period at 0.06 mg/L. However, the estimated equivalent amount of test material in solution was < 0.5 mg/L. Nevertheless, the test solutions were considered saturated with all soluble components of the test material, in test media and under test conditions.
Since the fish were exposed to a saturated solution of test material at the given loading rate, the effect concentrations were based on the loading rate (as recommended in OECD 23).
Under the conditions of the study, a saturated solution of the test material prepared at a loading rate of 100 mg/L in test media had no observable acute toxic effect on the fish. The 96-hour LL50 was therefore determined to be in excess of 100 mg/L. The results of the study demonstrate that the test material is not acutely toxic to fish up to the limit of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again