Registration Dossier
Registration Dossier
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Reaction mass of 6-(2-ethyl-6-methylanilino)-5-(4-{4-[ethyl(3-sulfobenzyl) amino] phenylazo} phenylazo)-4-hydroxynaphthalene-2-sulfonic acid, sodium and/or disodium salt(s) and 6-(2-ethyl-6-methylanilino)-5-(4-{4-[ethyl(4-sulfobenzyl) amino] phenylazo} phenylazo)-4-hydroxynaphthalene-2-sulfonic acid, sodium and/or disodium salt(s)
EC number: 475-080-7 | CAS number: -
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- Acid Navy RN-2682
- IUPAC Name:
- Acid Navy RN-2682
- Test material form:
- solid
Constituent 1
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- other: aerobic
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20 °C
- Dissolved oxygen:
- at least 8.5 mg/l
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol at concentrations of 5, 16, 50 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Details on results:
- Test item had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation of 3 hours in a limit test concentration of 1000 mg/l.
- Results with reference substance (positive control):
- EC50 (3h) = 14 mg/l
Applicant's summary and conclusion
- Conclusions:
- EC50 (3h) > 1000 mg/l
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