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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
Version / remarks:
GLP compliance:

Test material

Constituent 1
Reference substance name:
Acid Navy RN-2682
Acid Navy RN-2682
Test material form:

Study design

Oxygen conditions:
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
35 d
Initial test substance concentration
Initial conc.:
70.3 - 70.4 mg/L
Based on:
Details on study design:
Light conditions: test flasks were incubated in a dark room.
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
% degradation (DOC removal)
Sampling time:
28 d
Details on results:
In test flasks, containing test item and activated sludge (inoculum), fluctuating DOC concentrations were measured during the exposure period of 35 days. On exposure Day 35, the measured DOC concentrations were not significantly different from the initial mean DOC concentrations measured on Day 0. The fluctuating DOC concentrations are most likely due to adsorption and desorption of the test item on the activated sludge (inoculum).
This assumption is supported by the DOC concentrations of the test item measured on Day 0, which were below the aspired theoretical values.
Therefore, test item is classified as not biodegradable under the test conditions.
In the toxicity control, containing the test item, the reference item sodium benzoate and activated sludge (inoculum), the initial DOC decreased by 51 % within 14 days of exposure. Thus, according to the test guidelines the test item was not inhibitory to activated sludge at the tested concentration of 70 mg/l because degradation was >35 % within 14 days.

Points of degradation plot (test substance):
0 % degradation after 3 d
0 % degradation after 7 d
16 % degradation after 14 d
0 % degradation after 28 d

BOD5 / COD results

Results with reference substance:
The reference item sodium benzoate was completely (>90%) biodegraded within seven days of exposure, thus confirming suitability of the activated sludge.

Points of degradation plot:
100 % degradation after 3 d
100 % degradation after 7 d
100 % degradation after 14 d
99 % degradation after 28 d

Applicant's summary and conclusion

Interpretation of results:
under test conditions no biodegradation observed
Not biodegradable under test conditions.